Investigation of survivin gene polymorphism and serum survivin levels in patients with brain tumors

Kafadar, D. | Yaylim, I. | Kafadar, A.M. | Cacina, C. | Ergen, A. | Kaynar, M.Y. | İşbir, Turgay

Article | 2018 | Anticancer Research38 ( 10 ) , pp.5991 - 5998

Background/Aim: The single nucleotide polymorphism-31C/G identified in the survivin gene promoter seems to be associated with over-expression of survivin, an anti-apoptotic protein. In gliomas, increased survivin expression correlated with decreased survival. The aim of the study was to investigate whether survivin gene polymorphism associates with benign and malignant brain tumors and whether it affects survivin serum levels. Patients and Methods: Survivin polymorphism-31C>G was genotyped in 82 patients with brain tumors and 65 healthy controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sur . . .vivin levels were evaluated by enzyme-linked immuno sorbent assay (ELISA) in patients and controls. Results: Serum survivin levels in patients with malignant tumors were higher than patients with benign tumors (pG did not differ between patients and controls. Conclusion: Survivin promoter-31C>G gene polymorphism seems to be associated with serum survivin levels in brain tumors of different grades and histologies. © 2018 International Institute of Anticancer Research. All rights reserved Daha fazlası Daha az

Circulating microRNAs as novel biomarkers for atherosclerosis

Yilmaz, S.G. | Isbir, S. | Kunt, A.T. | İşbir, Turgay

Article | 2018 | In Vivo32 ( 3 ) , pp.561 - 565

Background/Aim: In this study, we determined the expression of selected circulating microRNAs (miRNA) and their potential roles as biomarkers in patients with atherosclerosis and a control group. Materials and Methods: In order to obtain insight into miRNA expression levels in atherosclerosis, we analyzed miRNA expression levels by real-time polymerase chain reaction (RT-PCR) in case (n=89) and healthy control (n=93) groups. Receiver operating characteristic curve analysis was performed to assess the diagnostic capability of miRNAs. Results: miRNA221 and miRNA221 expression levels were significantly lower in patients than controls ( . . .p=0.011 and p=0.004, respectively). Receiver operator curve analysis demonstrated that expression levels of miRNA221 [area under curve (AUC)=0.623, p=0.0086) and miRNA222 (AUC=0.654, p=0.0006) were significantly different between groups. There were positive correlations between miRNA122a and triglyceride (p=0.046) and very-low-density lipoprotein (p=0.029) levels. Conclusion: miRNA221 and miRNA222 could be convenient biomarkers for diagnosis of atherosclerosis Daha fazlası Daha az

Endothelial lipase gene polymorphism (584 C/T) in coronary artery patients among a Turkish population

Solim, L.A. | Gencan, I.A. | Çelik, B. | Ataacar, A. | Koç, U. | Büyükören, B. | Isbir, S.

Article | 2018 | In Vivo32 ( 5 ) , pp.1105 - 1109

Background/Aim: The endothelial lipase gene (LIPG) has a major role in regulating high density lipoprotein cholesterol (HDL-C), therefore this study investigated whether LIPG is associated with coronary artery disease (CAD) in a Turkish population. Materials and Methods: The LIPG (584 C/T) mutation was analyzed in 74 CAD patients and 73 controls. Results: There was a significant difference between the two groups regarding the mutant T allele frequencies (?2=0.456, p=0.020; 26.7% and 41.8% in patient and control groups, respectively) for 584 C/T. Even though the TT genotype was not significantly different, it had p=0.054 which suppor . . .ted our results. Conclusion: The endothelial lipase gene (584 C/T) T allele might be protective in association with coronary artery disease. Therefore, LIPG gene is related to risk for CAD in the Turkish population probably through altering HDL-C metabolism. © 2018 Institute of Electrical and Electronics Engineers Inc. All rights reserved Daha fazlası Daha az

The relationship between sepsis-induced immunosuppression and serum toll-like receptor 9 level

Atalan, N. | Acar, L. | Yapici, N. | Kudsioglu, T. | Ergen, A. | Yilmaz, S.G. | İşbir, Turgay

Article | 2018 | In Vivo32 ( 6 ) , pp.1653 - 1658

Background/Aim: Our aim was to determine serum TLR-9 levels in sepsis and evaluate the relationship between sepsis and serum TLR-9 levels. Materials and Methods: The study group consisted of 80 consecutive patients with sepsis and 100 healthy individuals. The demographic characteristics, co-morbidities and hemodynamic data of all patients were recorded. Results: TLR-9 serum levels in sepsis were statistically significantly lower compared to the control group. It was also seen that when the lactate level was >5 mmol/l in patients in the sepsis group, the serum TLR-9 levels were substantially higher. Conclusion: There is a relationshi . . .p between sepsis-induced immunosuppression and serum TLR-9 levels. The host immunity system can be activated by means of TLR-9-related systems, while hyperlactatemia may play a stimulating role in the re-activation of the immune system. © 2018 Institute of Electrical and Electronics Engineers Inc. All rights reserved Daha fazlası Daha az

Tumor necrosis factor-alpha (TNF-?-308 G>A) polymorphism in high-grade gliomas

Ozdogan, S. | Yaltirik, C.K. | Yilmaz, S.G. | Kaya, M. | Duzkalir, A.H. | Demirel, N. | İşbir, Turgay

Article | 2018 | In Vivo32 ( 2 ) , pp.287 - 289

Background/Aim: High-grade gliomas (HGG) consist of anaplastic oligoastrocytomas, anaplastic oligodendrogliomas, anaplastic astrocytomas and glioblastoma multiforme. The present study aimed to evaluate TNF-? -308 G>A polymorphism in a Turkish population. Patients and Methods: This was a prospective case-control study that included 45 patients with HGG and 49 healthy individuals. All patients were operated for intracranial tumors and the pathology results consist of high grade (Grade3 and 4) glial tumors. Results: No significant differences were found between the HGG and control groups in terms of the median age (p=0.898). There were . . . no significant differences with regard to gender (p=0.577). The TNF genotype frequency comparison between patients and controls was not statistically significant (p=0.598). Conclusion: TNF genotype frequency comparison between the patients and controls was not statistically significant in the Turkish population tested. However, further studies are needed to evaluate the genotype and phenotype correlations in large cohorts of various ethnicities. © 2018 International Institute of Anticancer Research. All Rights Reserved Daha fazlası Daha az

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