Sandal, S | Tuneva, J | Yılmaz, B | Carpenter, DO

Article | 2009 | CELL BIOCHEMISTRY AND FUNCTION27 ( 3 ) , pp.155 - 161

We investigated the effects of lipids oil thymocyte function. The effects of application of cholesterol or docosahexaenoic acid (DHA). a C22, omega-3 (n-3) polyunsaturated fatty acid (PUFA), on viability and intracellular calcium concentrations of acutely isolated mouse thymocytes were investigated using flow cytometry. Cholesterol (100 mu M) caused significant cell death after 30-60 min whether or not calcium was present in the medium. Cell death was associated with an elevation of intracellular calcium whether or not calcium was present in the extracellular medium. However, the elevation of calcium concentration was not responsibl . . .e for the cell death since calcium levels in the presence of ionomycin rose higher without significant cell death. DHA had similar actions but was more potent, causing significant cell death and elevation of calcium concentration within 5 min at 1 mu M. In the absence of extracellular calcium 1 mu M DHA caused 100% cell death within 15 min. Linolenic acid, a C18 omega-3 fatty acid also Caused cytotoxicity at low concentrations whether or not albumin was present, but omega-6 or saturated C22 fatty acids were much less effective. These observations demonstrate that thymocyte viability is very sensitive to acute exposure to low concentrations of omega-3 fatty acids. Copyright (c) 2009 John Wiley & Soils, Ltd Daha fazlası Daha az

Zortul, H | Yılmaz, B | Aricioglu, F

Conference Object | 2019 | EUROPEAN NEUROPSYCHOPHARMACOLOGY29 , pp.155 - 161

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Ethemoglu, MS | Arslan, I | Seker, FB | Ekimci, N | Duman, G | Yılmaz, B | Kilic, E

Conference Object | 2014 | ACTA PHYSIOLOGICA211 , pp.153 - 153

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Algul, S | Yılmaz, B | Ozcelik, O

Conference Object | 2015 | ACTA PHYSIOLOGICA215 , pp.93 - 93

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Agus, S | Atasayan, O | Sandal, S | Mete, F | Eyuboglu, S | Yılmaz, B

Conference Object | 2017 | ACTA PHYSIOLOGICA221 , pp.121 - 121

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Yaba, A | Agus, S | Yildirim, E | Erdogan, CS | Yılmaz, B

Article | 2020 | JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION , pp.121 - 121

Context: Oocyte and granulosa cells (GCs) have bidirectional communication and GCs play an important role in folliculogenesis and proliferation of GCs is very important for the development of ovulatory follicle. DNA double-strand breaks activate c-Abl protein tyrosine kinase and c-Abl has a functional role in repairement of DNA and control of telomere. Objective: In this study, we hypothesized that c-Abl has a regulative role on mTERT in mouse ovarian granulosa cells (GCs) and we aimed to detect c-Abl and mTERT interaction in mouse primary culture of GCs. Materials and methods: Mouse ovarian granulosa cell were cultured and siRNA-me . . .diated knockdown approach was used to knockdown c-Abl expression. Results: We showed c-Abl and mTERT immunolocalization in vivo and in vitro mouse GCs. c-Abl and mTERT were constitutively expressed in mouse granulosa cells and c-Abl presented more intense expression in granulosa cells than mTERT expression. The interaction of the c-Abl-mTERT is supported by the exhibition that c-Abl siRNA knockdown cells show decreased mTERT expression. We also present an interaction between c-Abl and mTERT by immunoprecipitation. In addition, our results indicated that the down-regulation of c-Abl was also accompanied by reduced expression of proliferating cell nuclear antigen (PCNA) in GCs. Conclusions: We suggest that mTERT may associate with the c-Abl in mouse GCs and the interactions between c-Abl and mTERT suggest a role for c-Abl in the regulation of telomerase function and proliferation in mouse granulosa cells Daha fazlası Daha az

Agus, S | Dinc, SE | Apaydin, A | Sandal, S | Vitrinel, A | Yılmaz, B

Conference Object | 2014 | ACTA PHYSIOLOGICA211 , pp.180 - 180

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Agus, S | Atasayan, O | Sandal, S | Mete, F | Eyuboglu, S | Yılmaz, B

Conference Object | 2016 | ACTA PHYSIOLOGICA218 , pp.61 - 61

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Ucar, AY | Aydin, MS | Agus, S | Gunalan, E | Yildirim, E | Yılmaz, B

Conference Object | 2017 | REPRODUCTIVE SCIENCES24 , pp.61 - 61

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Yılmaz, B | Vitrinel, A | Akin, Y | Erdag, GC | Agzikuru, T | Comert, S | Sadikoglu, S

Conference Object | 2008 | ACTA PAEDIATRICA97 , pp.214 - 214

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Agus, S | Civas, CC | Eyuboglu, S | Yavuz, Y | Baser, O | Topcu, IC | Yılmaz, B

Conference Object | 2019 | ACTA PHYSIOLOGICA227 , pp.138 - 139

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Yildirim, G | Attar, R | Ozkan, F | Ficicioglu, C | Karateke, A | Yılmaz, B | Yesildaglar, N

Conference Object | 2010 | BRITISH JOURNAL OF SURGERY97 , pp.138 - 139

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