Yencilek, F. | Kalkan, M. | Uzun, H. | Akkuş, E. | Öner, A. | Solok, V.

Article | 2003 | Turk Uroloji Dergisi29 ( 2 ) , pp.126 - 132

Introduction: Glycosaminogylicans (GAG) play as a promotor and inhibitor in some phases of stone formation. Sialic acid (SA) is a low-molecular weight aminosaccaride and found in the molecular structure of GAG and on the surface of many cells. Plasma SA level increases in inflamatory diseases, glomerulonephritis and metabolic abnormalities. Unfortunately, there are only a few studies investigating the relationship between SA level in urine and urological diseases. In this study, the relationship between calcium oxalate crystalization and free, total and complex sialic acid levels in urine and plasma has been investigated. Additional . . .ly, possible effect of potassium citrate on this relationship was also studied. Material and Methods: Thirty wistar female rats were included in this study. They were randomly divided in three groups. 0.12 ml of %5 ethylene glycole was dissolved in water and given to the rats in group 1 by feeding tube two times a day and every other day 0.5 microgram vitamin D3 was added to their drinking water to expose them hyperoxlacuric and hypercalciuric, respectively. Rats in group 2 were exposed to the same procedure as group1. Additionally, 5 mg/day K-Citrat was added to their diet. No special diet program was applied in group 3 (control group). At the end of 30 days, 24 hours urine samples were collected by using metabolic cages and blood samples were taken from each rat. Free and total SA levels were calculated by the technique of measuring thiobarbituric acid as mmol/liter (Warren's method). One way ANOVA test was applied for statistical analysis. Result: Calcium levels measured in plasma and urine were higher in group 1 and 2 than the control group. Similarly, oxalate levels in urine were also higher in group 1 and group 2., The differences were statistically significant when compared with the control group ( Daha fazlası Daha az

Yencilek, F. | Kalkan, M. | Uzun, H. | Akkuş, E. | Öner, A. | Solok, V.

Article | 2003 | Türk Üroloji Dergisi/Turkish Journal of Urology29 ( 2 ) , pp.126 - 132

Glikozaminoglikanlar taş oluşumunun bazı aşamalarında promoter, bazı aşamalarında ise inhibitor gibi davranmaktadır. Düşük molekül ağırlıklı bir aminosakkarid olan sialik asit (SA) glikozaminoglikanlanın yapısında bulunur. Kontrollü deney grupları oluşturarak kalsiyum-okzalat kristalizasyonu ile idrarda serbest ve bağlı SA ilişkisini ve potasyum sitratm bu ilişki üzerine etkisini araştırdık. Çalışmamızda Wistar cinsi dişi sıçanlardan 10'arlı 3 ayrı grup oluşturuldu. Grup l 'e 0.12 mi %5 etilen glikol l mi normal çeşme suyunda çözündürülerek 2x1 feeding tüple verilip hiperoksalürik ve günaşırı 0.5 mikrogram vitamin ds sularına karışt . . .ırılıp hiperkalsiürik hale getirildi. Grup 2, Grup l'den farklı olarak sularına 5mg/gün K-Sitrat eklendi. Grup 3'e normal beslemne yapıldı. Bütün sıçanlardan 30. günde metabolik kafesler kullanılarak 24 saatlik idrar ve kan örnekleri alındı. İdrarda serbest ve total SA düzeyleri Warren'in tarif ettiği tiobarbüritik asit tekniği ile standart eğrilere göre mmol/litre cinsinden hesaplandı. İstatistiksel analizde One way ANOVA test'i kullanıldı.Seram ve idrar kalsiyum, idrar okzalat düzeyleri grup l ve 2'de grup 3'e göre artmış olup istatistiksel anlamlı bulundu. Serum total ve bağlı SA düzeyi üç grupta normal sınırlar içerisinde fakat serbest SA grup l ve 2'de kontrol grubuna göre düşük bulundu ve bu istatistiksel anlamlı idi. İdrar total ve bağlı SA düzeyleri grup l ve 2'de grup 3'e göre azalmış bulundu ve bu istatistiksel anlamlı iken kendi aralarında istatistiksel anlamlılık yoktu. Her üç grup idrar serbest SA düzeyi arasında istatistiksel anlamlılık bulunmadı. Kristalüri ile idrar ve serum SA düzeyleri arasında anlamlı ilişki vardır. Potasyum sitrat'ın seram ve idrar SA üzerine anlamlı bir etkisi yoktur. Introduction: Glycosaminogylicans (GAG) play as a promoter and inhibitor in some phases of stone formation. Sialic acid (SA) is a low-molecular weight aminosaccaride and found in the molecular, structure of GAG and on the surface of many cells. Plasma SA level increases in inflammatory diseases, glomerulonephritis and metabolic abnormalities. Unfortunately, there are only a few studies investigating the relationship between SA level in urine and urological diseases. In this study, the relationship between calcium oxalate crystalization and free, total and complex sialic acid levels in urine and plasma has been investigated. Additionally, possible effect of potassium citrate on this relationship was also studied. Material and Methods: Thirty Wistar female rats were included in this study. They were randomly divided in three groups. 0.12 ml of %5 ethylene glycole was dissolved in water and given to the rats in group 1 by feeding tube two times a day and every other day 0.5 microgram vitamin D3 was added to their drinking water to expose them hypcroxlacuric and hypercalciuric, respectively. Rats in group 2 were exposed to the same procedure as group l Additionally, 5 mg/day K-Citrat was added to their diet. No special diet program was applied in group 3 (control group). At the end of 30 days, 24 hours urine samples were collected by using metabolic cages and blood samples were taken from each rat. Free and total SA levels were calculated by the technique of measuring thiobarbituric acid as mmol/liter (Warren's method). One way ANOVA test was applied for statistical analysis. Result: Calcium levels measured in plasma and urine were higher in group 1 and 2 than the control group. Similarly, oxalate levels in urine were also higher in group 1 and group 2., The differences were statistically significant when compared with the control group (p<0.05). Phisma complex and total SA levels were almost the same in all groups but free SA was significantly lower in group 1 and 2 than the controls (p<0.05). Low levels of total and complex SA in the urine were statistically significant in group 1 and 2 compared to the controls (p<0.05). In contrary, there was no significant difference between group 1 and 2. There was no statistical significance in urine free SA levels among 3 groups. Conclusion: In this experimental study, significant correlation is found between crystaluria and urine and plasma SA levels. In our opinion, SA can make a complex with crystals in urine and acts as a promoter in stone formation. Consequently, urine levels of SA can change in crystalluric cases. However, potassium citrate has not any significant effect on urine and plasma SA levels Daha fazlası Daha az