Cytoglobin inhibits migration through PI3K/AKT/mTOR pathway in fibroblast cells

Demirci, S. | Doğan, A. | Apdik, H. | Tuysuz, E.C. | Gulluoglu, S. | Bayrak, O.F. | Şahin, Fikrettin

Article | 2018 | Molecular and Cellular Biochemistry437 ( 01.02.2020 ) , pp.133 - 142

Cell proliferation and migration are crucial in many physiological processes including development, cancer, tissue repair, and wound healing. Cell migration is regulated by several signaling molecules. Identification of genes related to cell migration is required to understand molecular mechanism of non-healing chronic wounds which is a major concern in clinics. In the current study, the role of cytoglobin (CYGB) gene in fıbroblast cell migration and proliferation was described. L929 mouse fibroblast cells were transduced with lentiviral particles for CYGB and GFP, and analyzed for cell proliferation and migration ability. Fibroblas . . .t cells overexpressing CYGB displayed decreased cell proliferation, colony formation capacity, and cell migration. Phosphorylation levels of mTOR and two downstream effectors S6 and 4E-BP1 which take part in PI3K/AKT/mTOR signaling declined in CYGB-overexpressing cells. Microarray analysis indicated that CYGB overexpression leads to downregulation of cell proliferation, migration, and tumor growth associated genes in L929 cell line. This study demonstrated the role of CYGB in fibroblast cell motility and proliferation. CYGB could be a promising candidate for further studies as a potential target for diseases related to cell migration such as cancer and chronic wound treatment. © 2017, Springer Science+Business Media, LLC Daha fazlası Daha az

A Genome-Wide Analysis of mRNA Expression in Human Tooth Germ Stem Cells Treated with Pluronic P85

Ataei, A. | Solovyeva, V.V. | Poorebrahim, M. | Blatt, N.L. | Salafutdinov, I.I. | Şahin, Fikrettin | Rizvanov, A.A.

Article | 2016 | BioNanoScience6 ( 4 ) , pp.392 - 402

Human tooth germ stem cells (hTGSCs) originate from the neural crest and have a great potential to be used in stem cell therapies. Our group has previously shown that Pluronics interact with stem cells and affect their biological function. Pluronics block copolymer (P85), a potential drug delivery agent in the micelle form, which was shown to improve stem cell expansion. However, it is not known how P85 treatment affects the transcription profile of hTGSCs. In the present study, we found substantial changes in the expression of 252 genes in response to P85 treatment by using Illumina microarray. The gene enrichment was carried out u . . .sing database for annotation, visualization, and integrated discovery (DAVID) and the results classified in several biologically meaningful clusters. Using bioinformatics tools, we constructed a global regulatory network of P85-modulated genes associated with stem cell differentiation pathways and multi-drug resistance (MDR) processes. In conclusion, our results were compatible with many of the P85-mediated biological processes and may help us to gain a better molecular understanding of P85 biological function. © 2016, Springer Science+Business Media New York Daha fazlası Daha az

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