In this study, the double expression cassette plasmid, based on pBudCE4.1 vector encoding transcription factors SOX2 and OCT4 was constructed using standard gene engineering techniques. Expression of recombinant genes was confirmed by immunoblotting. It is shown that genetic modification of multipotent mesenchymal stromal cells (MMSC), isolated from human third molar tooth germs, with resulting recombinant plasmid increases the level of expression both, transcription factors SOX2 and OCT4 in the treated cells, and also transcription factor NANOG. Analysis of histological sections of subcutaneous Matrigel implants, containing fluores . . .cently labeled MMSC, showed that genetic modification had no effect on cell viability
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.