Guzelmeric, E. | Ristivojevic, P. | Vovk, I. | Milojkovic-Opsenica, D. | Yeşilada, Erdem

Article | 2017 | Journal of Pharmaceutical and Biomedical Analysis132 , pp.35 - 45

Chamomile tea composed of dried flower heads of Matricaria recutita L. (Asteraceae) is one of the most popular single ingredient herbal teas. Tea industries, spice shops or public bazaars are mostly supplied chamomile as a raw material via cultivation or through nature-picking. However, one of the drawbacks of nature-picking is adulteration. This could be either due to false authentication of the plant materials by ingenuous pickers or intentional/unintentional substitution with other flowers resembling to chamomile in appearance during harvesting. Therefore, quality control of raw chamomile materials before marketing should be care . . .fully considered not only by quantification of apigenin 7-O-glucoside (active marker) but also by fingerprinting of chemical composition. This work presents both quantification of apigenin 7-O-glucoside and chemical fingerprinting of commercial chamomile tea products obtained from different food stores and spice shops by a validated HPTLC method. In addition, HPTLC profiles of investigated chamomile tea samples were compared with HPLC method stated in the European Pharmacopoeia and it was found that HPTLC method was superior to HPLC method in the field of adulteration confirmation. Therefore, fingerprint profiles performed on the silica gel 60 NH2 F254s HPTLC plates combined with pattern recognition techniques of these marketed products were comparatively evaluated with wild and cultivar chamomile samples and also chamomile-like species from Asteraceae. Consequently, not chamomile tea bags but crude flowers sold on market were found to be adulterated with other plant materials. © 2016 Elsevier B.V Daha fazlası Daha az

Kupeli, E. | Tosun, A. | Yeşilada, Erdem

Article | 2007 | Journal of Ethnopharmacology113 ( 2 ) , pp.332 - 337

The n-hexane, diethylether, ethyl acetate and methanol extracts from roots, leaves, stems and flowers with young leaves of Daphne pontica L. (Thymelaeaceae) were investigated for their in vivo anti-inflammatory and antinociceptive activities. For the anti-inflammatory activity assessment, carrageenan-induced hind paw edema, PGE2-induced hind paw edema and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema models and for the antinociceptive activity, p-benzoquinone-induced abdominal constriction test were used. Only ethyl acetate extracts of the roots showed significant anti-inflammatory activity on carrageenan-induced (22.7 . . .-32.0% inhibition) and PGE2-induced hind paw edema (3.2-27.3% inhibition) as well as 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema (47.8-43.3% inhibition) models at 50 mg/kg dose without inducing any apparent gastric lesion or acute toxicity, whereas the other extracts were shown to be ineffective. In addition to roots, ethyl acetate extracts of the stems exhibited 19.5-29.9%; 5.3-23.9%; 36.6-28.1% inhibition on carrageenan-induced and PGE2-induced hind paw edema as well as 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema models, respectively. On the other hand, none of the extracts showed any significant antinociceptive activity. © 2007 Daha fazlası Daha az

Erdemoglu, N. | Akkol, E.K. | Yeşilada, Erdem | Caliş, I.

Article | 2008 | Journal of Ethnopharmacology119 ( 1 ) , pp.172 - 178

Ethnopharmacological relevance: Rhododendron ponticum L. (Ericaceae) is used for the treatment of inflammatory diseases and to alleviate rheumatic pain and against toothache in Turkish traditional medicine. Aim of the study: To evaluate the anti-inflammatory and antinociceptive effects of Rhododendron ponticum leaves using in vivo models, and isolation and chemical characterization of the biologically active constituents through bioassay-guided fractionation procedures. Material and Methods: Carrageenan-induced hind paw edema model was used for anti-inflammatory activity and p-benzoquinone induced abdominal contractions model for th . . .e antinociceptive activity assessment. Results: The ethylacetate fraction displayed marked anti-inflammatory (28.4-40.7% inhibition) and antinociceptive (50.7% inhibition) effects as compared to reference compounds. Through bioassay-guided fractionation and isolation procedures flavonol glycosides [a mixture of hyperoside and isoquercitrin (1) and quercitrin (2)] along with one flavanone glycoside [6-C-glycosylnaringenin (3)] were isolated as the active ingredients of ethylacetate extract against carrageenan-induced edema and p-benzoquinone-induced writhes and their structures were elucidated by spectral techniques. 1 and 2 also showed a significant anti-inflammatory activity against 12-O-tetradecanoyl-13-acetate (TPA)- induced mouse ear edema model. Conclusion: Results of the present study supported the utilization of the plant in Turkish folk medicine and revealed that flavones are the major anti-inflammatory and antinociceptive principles of the leaves. © 2008 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Süntar, I. | Küpeli Akkol, E. | Keles, H. | Yeşilada, Erdem | Sarker, S.D.

Article | 2013 | Journal of Ethnopharmacology149 ( 1 ) , pp.103 - 110

Ethnopharmacological relevance In Turkish traditional medicine, the flowers of Helichrysum graveolens (Bieb.) Sweet (Asteraceae) have been used for the treatment of jaundice, for wound-healing and as a diuretic. Aim of the study In order to find scientific evidence for the traditional utilization of this plant in wound-healing, the effect of the plant extract was investigated by using in vivo and in vitro experimental models. Then through bioassay-guided fractionation procedures active wound-healing component(s) was isolated and its possible role in the wound-healing process was also determined. Material and methods The linear incis . . .ion and the circular excision wound models were applied in order to evaluate in vivo wound-healing potential of Helichrysum graveolens. Anti-inflammatory and antioxidant activities, which are known to involve in wound-healing process, were also assessed by the Whittle method and the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assay, respectively. The total phenolic content of the crude extract and solvent fractions was estimated to find correlation between the phenolic content and the antioxidant activity. Combined application of the chromatographic separation techniques on sephadex and silica gel columns, and bioassay techniques have yielded the active wound-healing principle of Helichrysum graveolens. Moreover, in vitro inhibitory effect of active principle on hyaluronidase, collagenase and elastase enzymes were investigated to explore the activity pathways. Results The 85% methanol (MeOH) extract of Helichrysum graveolens flowers displayed significant wound-healing, anti-inflammatory and antioxidant activities. Then the crude extract was partitioned by successive solvent extractions, in increasing polarity, to give five solvent fractions. Among the solvent fractions, the ethyl acetate (EtOAc) fraction exerted the highest activity. The EtOAc fraction was further subjected to chromatographic separations to yield active constituent and its structure was elucidated to be apigenin by spectrometric methods. Further in vivo and in vitro assays revealed that apigenin was one of the components responsible for the wound-healing effect of the plant remedy and also found to possess significant anti-inflammatory, antioxidant, anti-hyaluronidase and anti-collagenase activities. Conclusion Present study supported the traditional use of Helichrysum graveolens flowers for wound-healing and through bioassay-guided fractionation procedures from the crude extract apigenin was isolated as one of the active components. © 2013 Elsevier Ireland Ltd Daha fazlası Daha az

Koca, U. | Süntar, I.P. | Keles, H. | Yeşilada, Erdem | Akkol, E.K.

Article | 2009 | Journal of Ethnopharmacology126 ( 3 ) , pp.551 - 556

Aim of the study: Several Centaurea species (Asteraceae) are used in Turkish folk medicine to alleviate pain and inflammatory symptoms in rheumatoid arthritis, high fever, head ache and for wound healing. Particularly, the aerial part of Centaurea iberica Trev. ex Spreng. has been practiced on wounds for healing. In order to evaluate the anti-inflammatory and wound healing activities of the plant, extracts were prepared with variety of solvents: hexane, chloroform, ethyl acetate and aqueous methanol (85%) from the aerial parts of Centaurea iberica. Materials and methods: The incision by using tensiometer and excision models were use . . .d in order to assess the effect of the plant extracts on wound healing in mice and rats. Results were also evaluated histopathologically. In vivo inhibitory effect of the extracts on acetic acid-induced increase in capillary permeability was studied for the assessment of anti-inflammatory activity. Results: The wound healing effect was comparatively evaluated with a reference ointment Madecassol®. Noteworthy wound healing activity was observed for the ointment formulation prepared with 1% methanol extract. The results of histopathological evaluation supported the outcome of both incision and excision wound models. Moreover, the methanol extract exerted remarkable wound healing activity and also demonstrated a significant and dose-dependent anti-inflammatory activity. Conclusion: The experimental study revealed that Centaurea iberica displays remarkable wound healing and anti-inflammatory activity. © 2009 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Deliorman Orhan, D. | Hartevioglu, A. | Küpeli, E. | Yeşilada, Erdem

Article | 2007 | Journal of Ethnopharmacology112 ( 2 ) , pp.394 - 400

The aqueous and ethanol extracts of Rosa canina L. (Rosaceae) fruits and the fractions prepared from the latter were investigated for their anti-inflammatory and antinociceptive activities in several in vivo experimental models. The ethanolic extract was shown to possess significant inhibitory activity against inflammatory models (i.e., carrageenan-induced and PGE1-induced hind paw edema models, as well as on acetic acid-induced increase in a capillary permeability model) and on a pain model based on the inhibition of p-benzoquinone-induced writhing in mice. Hexane, chloroform, ethylacetate, n-butanol and the remaining water fractio . . .ns were obtained through bioassay-guided fractionation. Ethylacetate and n-butanol fractions displayed potent anti-inflammatory and antinociceptive activities at a dose of 919 mg/kg without inducing acute toxicity. Further attempts to isolate and define the active constituent(s) were inconclusive, possibly due to the synergistic interaction of components in the extract. © 2007 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Küpeli, E. | Yeşilada, Erdem

Article | 2007 | Journal of Ethnopharmacology112 ( 3 ) , pp.524 - 530

Extracts obtained from the leaves and branches of various Cistus species have been used worldwide as folk remedy for the treatment of various inflammatory ailments including rheumatism and renal inflammations. Effects of the extracts and fractions from the leaves with non-woody branches of Cistus laurifolius L. (Cistaceae) were studied using two in vivo models of inflammation in mice. Model one was based on observed potent inhibitory activity against carrageenan-induced hind paw oedema and the second model used was acetic acid-induced, increased vascular permeability model. Through bioassay-guided fractionation and isolation procedu . . .res three flavonoids; 3-O-methylquercetin (1), 3,7-O-dimethylquercetin (2) and 3,7-O-dimethylkaempferol (3) were isolated as the main active ingredients from the ethanol extract. Later on, these flavonoids were shown to possess potent antinociceptive activity, which was assessed through inhibition of p-benzoquinone-induced writhing reflex. Results of the present study have clearly supported the utilization of Cistus laurifolius in Turkish traditional medicine. Subsequently, three flavonoids were shown to have strong antinociceptive and anti-inflammatory activities, per os without inducing any apparent acute toxicity as well as gastric damage. © 2007 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Yeşilada, Erdem

Review | 2008 | Drugs of the Future33 ( 8 ) , pp.673 - 680

Despite recent developments in computational and chemical techniques, traditional medicines are considered among the most reliable lead sources for the discovery of new drugs. A vast wealth of knowledge has been accumulated in Turkey as traditional medicinal heritage due to the rich cultural and plant diversity. Ethnopharmacological investigations have been carried out for the scientific evaluation of this information by our research team. Using in vivo bioassay- or in vitro activity-guided fractionation and isolation procedures, the active ingredients of several traditional remedies have been determined and defined chemically. In t . . .his review, several examples of the diverse pharmacological activity profile of these natural active ingredients will be discussed, i.e., sesquiterpene lactones with antiulcerogenic and antiviral activities; flavonoids with antiinflammatory, antinociceptive, antiulcerogenic, anti-Helicobacter pylori, antidiabetic and antihepatotoxic activity; and lignans with antiinflammatory, antinociceptive and antiulcerogenic activity. The author also draws attention to the discovery of multitargeted drug molecules for rational therapy. Copyright 2008 Prous Science, S.A.U. or its licensors. All rights reserved Daha fazlası Daha az

Özüdoru, B. | Akaydin, G. | Erik, S. | Yeşilada, Erdem

Article | 2011 | Journal of Ethnopharmacology137 ( 1 ) , pp.85 - 98

https://hdl.handle.net/20.500.11831/828

Köksal, M. | Gökhan, N. | Küpeli, E. | Yeşilada, Erdem | Erdogan, H.

Article | 2005 | Archiv der Pharmazie338 ( 02.03.2020 ) , pp.117 - 125

The synthesis of a novel series of Mannich bases of 5-/6-acyl-5-methyl-2- benzoxazolinones has been described. The structures attributed to compounds 2a, 3a, 4a, 4b, 9a, 9b, 5a-5g, 6a-6g, 1Oa, 1Og, 11a, 11g have been elucidated using IR and 1H NMR spectroscopic techniques besides elemental analysis. The compounds have been evaluated for their in vivo analgesic and antiinflammatory activities using the p-benzoquinone-induced writhing test and the carrageenan hind paw oedema test in mice, respectively. In addition, the ulcerogenic effects of the compounds were determined. Among the tested derivatives most promising results were obtain . . .ed for the compounds bearing a 6-(4-chlorobenzoyl) at C-6 position and 2-/4-fluorophenyl at C-3 position of 2-benzoxazolinone ring (11c, 11d). © 2005 WILEY-VCH Verlag GmbH & Co. KGaA Daha fazlası Daha az

Olate, V.R. | Pertino, M.W. | Theoduloz, C. | Yeşilada, Erdem | Monsalve, F. | González, P. | Schmeda-Hirschmann, G.

Article | 2012 | Planta Medica78 ( 4 ) , pp.362 - 367

Starting from the diterpene (4S,9R,10R) methyl 18-carboxy-labda-8,13(E)- dien-15-oate (PMD) and its 8(9)-en isomer [PMD 8(9)-en], 11 amides were prepared and assessed for a gastroprotective effect in the ethanol/HCl-induced gastric lesions model in mice. Basal cytotoxicity of the compounds was determined on the following human cell lines: normal lung fibroblasts (MRC-5), gastric epithelial adenocarcinoma (AGS), and hepatocellular carcinoma (Hep G2). All compounds are described for the first time. At the single oral dose of 0.1 mg/kg, compounds 1, 10, and 11 presented a strong gastroprotective effect, at least comparable with that of . . . the reference compound lansoprazole at 1 mg/kg, reducing gastric lesions by 76.7, 67.7, and 77.2 %, respectively. The leucyl amide methyl ester 3, tryptophanyl amide methyl ester 5, and benzyl amide 6 of PMD presented a selective basal cytotoxicity on Hep G2 cells with IC 50 values of 136.8, 105.3, and 94.2 µM, respectively, while the IC 50 values towards AGS cells were 439.5, 928.0, and 937.3 µM, respectively. The three compounds did not affect fibroblast viability with IC 50 values > 1000 µM. Compounds 7, 8, 10, and 11 showed no toxic effect against the three selected cell lines. © Georg Thieme Verlag KG Stuttgart Daha fazlası Daha az

Parildar, H. | Serter, R. | Yeşilada, Erdem

Review | 2011 | Journal of the Pakistan Medical Association61 ( 11 ) , pp.1116 - 1120

This study reports a literature review aimed to analyse various studies related to the use of phytotherapy in diabetes mellitus in Turkey in order to provide additional information for healthcare professionals. The incidence of Diabetes Mellitus is rising and many of the diabetics frequently use herbal treatments along with modern medical treatment for glycaemic control and/or improve their well-being. Several electronic databases (such as Medline and Pubmed) were searched for 1990-2010 period (till May, 2010) and 33 related articles were analysed. Many studies-mostly animal trials- have been conducted in this field. Among the herbs . . . most-commonly used along with modern medical therapies and also in folkloric medicine, we searched for bitter melon, cinnamon, fenugreek, olive leaf, black seed and white mulberry. Studies conducted in this field have produced conflicting results and, the necessity to conduct randomized, placebo-controlled clinical human studies to develop new drugs from herbs, as in the case of metformin, still remains important. Besides, further studies are required to address the issues of standardization and quality control of existing preparations. More importantly, healthcare professionals caring for diabetic patients need to be aware of phytotherapy to incorporate phytomedicine into their practices and should undertake more responsibility in relation to these kind of therapies that are commonly-used throughout the world Daha fazlası Daha az