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Donmez, MF | Şahin, Fikrettin | Elkoca, E

Article | 2013 | ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS12 ( 4 ) , pp.139 - 151

Bean diseases caused by different pathogens reduce important yield and quality of beans in different bean growing regions in both Turkey and the other bean producing countries. In the present study, bacterial pathogens in the species of Pseudomonas savastanoi pv. phaseolicola (Burkholder) Garden et al. and Xanthomonas axonopodis pv. Phaseoli (Smith) Vauterin et al. causing economically important disease on bean plants growing in the commercial fields of Erzurum and Erzincan provinces located in the Eastern Anatolia region of Turkey has been isolated and identified. Totally thirty-six bean genotypes and two commercial cultivars commo . . .nly grown in the region have been screened for resistance to these pathogens both in greenhouse and field condition during 2001-2002. Disease severity in the field condition reduced seed quality and quantity of bean. Among the thirty-eight genotypes tested, only 36K was found to be resistant to both of the pathogens Daha fazlası Daha az

Bacterial application for treatment of clogged emitters in drip irrigation systems as an environmentally friendly method

Eroglu, S | Şahin, Fikrettin | Sahin, U | Anapali, O

Conference Object | 2009 | NEW BIOTECHNOLOGY25 , pp.139 - 151

Kotan, R. | Şahin, Fikrettin | Demirci, E. | Eken, C.

Erratum | 2011 | Biological Control59 ( 3 ) , pp.139 - 151

[No abstract available]

Plant growth promoting rhizobacteria as alleviators for soil degradation

Turan, M. | Esitken, A. | Şahin, Fikrettin

Book Part | 2011 | Bacteria in Agrobiology: Stress Management , pp.41 - 63

Soil degradation refers to decline in the soil's productivity through deterioration of its physical, chemical, and biological properties. The most important processes and causes of degradation are water-wind erosion, salinization, alkalinization, acidification, and leaching and soil pollution. The rate of soil degradation is directly related to unsuitable land use. While growers routinely use physical and chemical approaches to manage the soil environment to improve crop yields, the application of microbial products for this purpose is less common. However, plant growth promoting rhizobacteria (PGPRs) can prevent the deleterious eff . . .ects of one or more stressors from the environment. These beneficial microorganisms can be a significant component of management practices to achieve the attainable yield in degraded soil. In such soils, the natural role of stress-tolerant PGPRs in maintaining soil fertility is more important than in conventional agriculture. Besides their role in metal detoxification/removal, salinization, and acidification, rhizobacteria also promote the growth of plants by other mechanisms such as production of growth promoting substances and siderophores. Remediation with PGPRs is called bioremediation in degraded soil and is another emerging low-cost in situ technology (Cohen et al. Int J Green Energy 3:301-312, 2004) employed to remove or alleviate pollutants, salinity, and acidification stress from the degraded land. The efficiency of bioremediation can be enhanced by the judicious and careful application of appropriate heavy metal, salinity, acidity tolerant, and plant growth promoting rhizobacteria including symbiotic nitrogen-fixing organisms. This review presents the results of studies on the recent developments in the utilization of PGPR for direct application in soils degraded with heavy metals, salinity, and acidity under a wide range of agroecological conditions with a view to restore degraded soils and consequently, promote crop productivity in degraded soils across the globe and their significance in bioremediation. © Springer-Verlag Berlin Heidelberg 2012. All rights are reserved Daha fazlası Daha az

Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth

Demirel, S. | Yalvac, M.E. | Tapsin, S. | Akyuz, S. | Ak, E. | Cetinel, S. | Şahin, Fikrettin

Article | 2016 | SpringerPlus5 ( 1 ) , pp.41 - 63

Treatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the r . . .ats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases. © 2016, The Author(s) Daha fazlası Daha az

Erratum: Bone marrow mesenchymal stem cells enhance bone formation in orthodontically expanded maxillae in rats (The Angle Orthodontist (2015) 85:3 (394-399))

Ekizer, A. | Yalvac, M.E. | Uysal, T. | Sonmez, M.F. | Şahin, Fikrettin

Erratum | 2015 | Angle Orthodontist85 ( 5 ) , pp.41 - 63

[No abstract available]

Antimicrobial properties of zeolite-X and zeolite-A ion-exchanged with silver, copper, and zinc against a broad range of microorganisms

Demirci, S. | Ustaoglu, Z. | Yilmazer, G.A. | Şahin, Fikrettin | Baç, N.

Article | 2014 | Applied Biochemistry and Biotechnology172 ( 3 ) , pp.1652 - 1662

Zeolites are nanoporous alumina silicates composed of silicon, aluminum, and oxygen in a framework with cations, water within pores. Their cation contents can be exchanged with monovalent or divalent ions. In the present study, the antimicrobial (antibacterial, anticandidal, and antifungal) properties of zeolite type X and A, with different Al/Si ratio, ion exchanged with Ag+, Zn2+, and Cu2+ ions were investigated individually. The study presents the synthesis and manufacture of four different zeolite types characterized by scanning electron microscopy and X-ray diffraction. The ion loading capacity of the zeolites was examined and . . .compared with the antimicrobial characteristics against a broad range of microorganisms including bacteria, yeast, and mold. It was observed that Ag+ ion-loaded zeolites exhibited more antibacterial activity with respect to other metal ion-embedded zeolite samples. The results clearly support that various synthetic zeolites can be ion exchanged with Ag+, Zn2+, and Cu2+ ions to acquire antimicrobial properties or ion-releasing characteristics to provide prolonged or stronger activity. The current study suggested that zeolite formulations could be combined with various materials used in manufacturing medical devices, surfaces, textiles, or household items where antimicrobial properties are required. © 2013 Springer Science+Business Media Daha fazlası Daha az

PCR detection of Brucella abortus in cow milk samples collected from Erzurum, Turkey

Arasoğlu, Tülin | Güllüce, Medine | Özkan, Hakan | Adıgüzel, Ahmet | Şahin, Fikrettin

Article | 2013 | Turkish Journal of Medical Sciences43 ( 4 ) , pp.501 - 508

Aim: In this study, we designed and used 2 different type-specific polymerase chain reaction (PCR) methods for the detection of Brucella abortus. Materials and methods: There were 2 different primer sets (B4/B5 and AF/AR) selected and used for the amplification of 2 different genes that are present in all biovars of Brucella species, including bcsp31, encoding 31-kDa immunogenic cell surface proteins, and omp25, which is known to be one of the virulence factors encoding outer membrane proteins of 26–23 kDa. Results: The results showed that 273 (81.7%) and 317 (94.9%) out of 334 milk samples were positive for brucellosis, a . . .s detected by either both or one of the primer sets used in the present study, respectively. The detection limit of PCR assays for Brucella in milk samples was determined as 5 pg DNA for both of the primer sets. Conclusion: These results suggest that the use of the specific PCR assay with the primer sets used in the study is a rapid, reliable, and accurate technique in comparison to traditional and conventional methods for the detection and diagnosis of Brucella spp. in milk samples Daha fazlası Daha az

In vitro antimicrobial and antioxidant activities of methanol and hexane extract of Astragalus species growing in the Eastern Anatolia Region of Turkey

Adıgüzel, Ahmet | Sökmen, Münevver | Özkan, Hakan | Ağar, Güleray | Güllüce, Medine | Şahin, Fikrettin

Article | 2009 | Turkish Journal of Biology33 ( 1 ) , pp.65 - 71

Bu çalışma Türkiye’de Doğu Anadolu’da bulunan Erzurum yöresinde yetişen bazı Astragalus türlerinin topraküstü ve kök kısımlarının metanol ve hekzan ekstraktlarının antimikrobiyal ve antioksidan aktivitelerinin araştırılması için yapılmıştır. Astragalus türlerinin topraküstü kısımlarından elde edilen metanol ekstraktı 68,8-400,4 ?g/ml konsantrasyonları aralığında % 50 inhibisyon ile hafif düzeyde serbest radikal süpürücü etki göstermiştir. Astragalus türlerinin topraküstü kısımlarının hekzan/diklorometan ekstraktları serbest radikal süpürücü etki göstermemişlerdir. Nonpolar ekstraktların linoleik asit oksidasyonu inhibisyon özellikle . . .rinin polar metanolik ekstraktlarınkinden yüksek olduğu görülmektedir. Kök kısımlarının metanol ekstraktları hafif antioksidanlar iken, A. microcephalus Willd., A. macrocephalus Willd., A. erinaceus Fisch. & Mey. ex Fischer, A. psoraloides Lam. ve A. argyroides Becker ex Stapf. türlerinin non-polar ekstraktları DPPH testinde son derece aktif olarak görüldüler. IC50 değerleri sırası ile 35,2 ?g/ml, 21,0 ?g/ml, 22,0 ?g/ml, 20,3 ?g/ml ve 38,0 ?g/ml olarak tespit edildi. ?–karoten-linoleik asit sistemi içinde, çalışılan Astragalus türleri arasında en güçlü inhibisyon etkisini A. psoraloides Lam. türünden elde edilen ekstrakt gösterdi. Buna ek olarak, disk difüzyon yöntemi ile Astragalus türlerinin in vitro antibakteriyel, antikandidal ve antifungal aktiviteleri de çalışılmıştır. Sonuçlar ekstraktların hiçbirinin çalışılan mikroorganizmalar üzerinde antimikrobiyal etkisinin olmadığını göstermiştir. This study was conducted to evaluate the antimicrobial and antioxidant activities of the hexane and methanol extracts of the aboveground parts and roots of some Astragalus species grown in the vicinity of Erzurum, located in the eastern Anatolia, Turkey. The methanol extract, which was obtained from the aboveground parts of Astragalus species, exhibited mild free radical scavenging activity providing 50% inhibition at the concentrations between 68.8 and 400.4 μg/ml. The hexane/dichloromethane extracts of the aboveground parts of Astragalus species did not show any free radical scavenging activity. However, the linoleic acid oxidation inhibition ability of the non-polar extracts seems to be higher than that of the polar methanolic extracts. The methanol root extracts were mild antioxidants but the non-polar root extracts of A. microcephalus Willd., A. macrocephalus Willd., A. erinaceus Fisch. & Mey. ex Fischer, A. psoraloides Lam., and A. argyroides Becker ex Stapf. were extremely active in DPPH test. IC50 values were 35.2 μg/ml, 21.0 μg/ml, 22.0 μg/ml 20.3 μg/ml, and 38.0 μg/ml, respectively. In the case of β–carotene-linoleic acid system, the extract obtained from A. psoraloides Lam. exhibited strong inhibition among studied Astragalus species. Furthermore, the extracts, which were obtained from the tested Astragalus species, were evaluated for their possible in vitro antibacterial, anticandidal, and antifungal activities based on disk diffusion assay. The results suggested that none of the extracts has antimicrobial activity on the tested microorganisms Daha fazlası Daha az

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