Akkol, E.K. | Yeşilada, E. | Güvenç, A.

Article | 2008 | Journal of Ethnopharmacology116 ( 2 ) , pp.251 - 257

Erica L. species (Ericaceae) have been popularly used as antirheumatic, diuretic, astringent and treatment of urinary infections. In order to evaluate this information, anti-inflammatory and antinociceptive activities of different extracts prepared with methanol, chloroform, ethyl acetate, n-butanol and water from the aerial parts of Erica arborea L., Erica manipuliflora Salisb., Erica bocquetii (Peşmen) P.F. Stevens and Erica sicula Guss. subsp. libanotica (C.&W. Barbey) P.F. Stevens (Ericaceae) of Turkish origin were investigated by using in vivo methods. For the anti-inflammatory activity, carrageenan-induced hind paw edema m . . .odel, PGE2-induced hind paw edema model, and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema model and for the antinociceptive activity p-benzoquinone-induced writhing test in mice were employed. The ethyl acetate extracts of Erica arborea (EAE), Erica bocquetii (EBE) and Erica manipuliflora (EME) exhibited notable inhibition against carrageenan-induced (24.1-32.3%, 23.8-36.1%, 29.2-35.1%, respectively) and PGE2-induced (21.2-37.7%, 6.8-29.7%, and 6.2-34.1%, respectively) hind paw edema as well as TPA-induced mouse ear edema models in mice, while the ethyl acetate extract of Erica sicula subsp. libanotica (ESE) (10.7-29.7%) displayed potent anti-inflammatory activity only on the PGE2-induced hind paw edema model. However, the remaining extracts were found to be inactive against inflammatory models. Same extracts, i.e., EAE, EBE and EME were also found to exhibit remarkable antinociceptive activity in p-benzoquinone-induced abdominal constriction test at a dose of 100 mg/kg (46.5%, 27.7% and 36.3%, respectively). © 2007 Daha fazlası Daha az

Koca, U. | Süntar, I.P. | Keles, H. | Yeşilada, Erdem | Akkol, E.K.

Article | 2009 | Journal of Ethnopharmacology126 ( 3 ) , pp.551 - 556

Aim of the study: Several Centaurea species (Asteraceae) are used in Turkish folk medicine to alleviate pain and inflammatory symptoms in rheumatoid arthritis, high fever, head ache and for wound healing. Particularly, the aerial part of Centaurea iberica Trev. ex Spreng. has been practiced on wounds for healing. In order to evaluate the anti-inflammatory and wound healing activities of the plant, extracts were prepared with variety of solvents: hexane, chloroform, ethyl acetate and aqueous methanol (85%) from the aerial parts of Centaurea iberica. Materials and methods: The incision by using tensiometer and excision models were use . . .d in order to assess the effect of the plant extracts on wound healing in mice and rats. Results were also evaluated histopathologically. In vivo inhibitory effect of the extracts on acetic acid-induced increase in capillary permeability was studied for the assessment of anti-inflammatory activity. Results: The wound healing effect was comparatively evaluated with a reference ointment Madecassol®. Noteworthy wound healing activity was observed for the ointment formulation prepared with 1% methanol extract. The results of histopathological evaluation supported the outcome of both incision and excision wound models. Moreover, the methanol extract exerted remarkable wound healing activity and also demonstrated a significant and dose-dependent anti-inflammatory activity. Conclusion: The experimental study revealed that Centaurea iberica displays remarkable wound healing and anti-inflammatory activity. © 2009 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Orhan, I. | Küpeli, E. | Şener, B. | Yeşilada, Erdem

Article | 2007 | Journal of Ethnopharmacology109 ( 1 ) , pp.146 - 150

Studies on four extracts prepared with petroleum ether, chloroform, ethyl acetate and methanol as well as the alkaloid fraction from the aerial parts of Lycopodium clavatum L. of Turkish origin using acetic acid-induced increase in capillary permeability assessment in mice revealed that only the chloroform extract and the alkaloid fraction displayed marked anti-inflammatory effect at a dose of 500 mg/kg having percentage of inhibition 24.3 and 32.1, respectively, as compared to indomethacin, which exhibited 44.6% of inhibition at 10 mg/kg dose. Bioassay-guided fractionation of the alkaloid fraction of Lycopodium clavatum revealed th . . .at the alkaloidal-type of compounds might possibly be responsible for the anti-inflammatory activity of the extract, which supports the folk medicinal utilization of the plant. Gas chromatographic-mass spectrophotometric analysis of the active alkaloid fraction revealed that lycopodine (84.5%) is the major component. © 2006 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Yücel, A. | Kan, Y. | Yeşilada, Erdem | Akın, O.

Article | 2017 | Journal of Ethnopharmacology196 , pp.236 - 241

Ethnopharmacological relevance Topical formulations such as oily extracts or ointments prepared with the flowering aerial parts of St. John's wort (Hypericum perforatum L., Hypericaceae) have been used in the management of a wide range dermatological problems including superficial wounds and burns, bruises, contusions and many others in the worldwide traditional medicines. Aim of the study This is the first case study reporting the beneficial effects of an oily extract of St. John's wort in the treatment of pressure sores in a intensive care unit (ICU) patient. Material and methods The oily extract of St. John's wort was applied to . . .a volunteer patient at ICU daily for forty successive days for wound care and treatment. Healing status was monitored macroscopically by measuring the wound size and stages at certain intervals as well as histopathological evaluation of the tissue sections taken at the initial and final dates of treatment. Results Evaluation of the results obtained from the macroscopical and histopathological experimentation have shown that oily extract of St. John's wort provided significant efficacy for the treatment of pressure sore wounds. Conclusion St. John's wort oily extract may be suggested as a cost-effective option for the prevention or treatment of pressure sores in ICU patients. © 2016 Elsevier Ireland Lt Daha fazlası Daha az

Küpeli Akkol, E. | Kirmizibekmez, H. | Küükboyaci, N. | Gören, A.C. | Yeşilada, Erdem

Article | 2012 | Journal of Ethnopharmacology139 ( 2 ) , pp.527 - 532

Ethnopharmacological relevance: The fresh leaves of Laurocerasus officinalis Roem. (Rosaceae) are externally used against pain and feverish symptoms in Turkish folk medicine. Aim of the study: Effects of the extracts, fractions and isolated compounds from the leaves of L. officinalis were investigated using in vivo models of inflammation and pain in mice. Methods: The crude ethanolic extract from the leaves of plant was sequentially fractionated into five subextracts; explicitly, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and remaining water extracts. Further studies were carried out on the most active EtOAc subextract . . .was further subjected to fractionation through column chromatography. For the anti-inflammatory activity, carrageenan-induced hind paw edema and acetic acid-induced increase in capillary permeability models, and for the antinociceptive activity p-benzoquinone-induced writhing test in mice were employed. Results: Ethanolic extract of the leaves was shown to possess significant inhibitory activity in the assay methods without inducing any gastric damage. Through bioassay-guided fractionation and isolation procedures three phenolic compounds, 2-O-ß-d-glucopyranosyl-2- hydroxyphenyl-acetic acid (1), kaempferol-3-O-ß-d-xylopyranosyl-(1›2)- O-ß-d-glucopyranoside (2) and (+)-catechin (3) were isolated from the active fraction and their structures were elucidated by spectral techniques (1D and 2D NMR, ESIMS). Conclusion: The experimental data verified that Laurocerasus officinalis leaves displayed remarkable anti-inflammatory and antinociceptive activity. © 2011 Elsevier Ireland Ltd Daha fazlası Daha az

Charehsaz, M. | Reis, R. | Helvacioglu, S. | Sipahi, H. | Guzelmeric, E. | Acar, E.T. | Aydin, A.

Article | 2016 | Journal of Ethnopharmacology194 , pp.506 - 512

Ethnopharmacological relevance Styrax liquidus is a resinous exudate (balsam) obtained from the wounded trunk of the Liquidambar orientalis Mill. (Hamamelidaceae). Styrax has been used for treatment of various ailments in Turkish folk medicine such as skin problems, peptic ulcers, nocturnal enuresis, parasitic infections, antiseptic or as expectorant. Aim of study In spite of frequent use of styrax in Turkish folk medicine as well as once as a stabilizer in perfumery industry, negative reports have been noticed by the international authority for restriction its use based on some limited evidences from an in vitro study. The aim of t . . .he present study was to evaluate the genotoxic and cytotoxic potential of styrax and its ethanolic extract using in vivo and in vitro assays, as well as an antimutagenic assay and also to determine its phenolic constituents with chromatographic analysis. Materials and methods In vitro mutagenicity and antimutagenicity of styrax and its ethanolic extract were evaluated by Ames test performed on Salmonella TA98 and TA100 strains with and without metabolic activation (10– 30,000 µg/plate). The genotoxicity was also studied in vivo by chromosomal aberrations assay on bone marrow of Balb C mice with different its concentrations (500–2000 mg/kg body weight). Cytotoxicity has been evaluated by the MTT assay using L929 cell line. Its phenolic constituents were determined by HPLC analysis. Results Genotoxicological investigations of styrax or its ethanolic extract showed that none of the tested concentrations induced a significant increase in the revertant number of TA98 and TA100 strains with or without metabolic activation, indicating no mutagenicity to the tested strains. Also results indicated that up to 2000 mg/kg body weight, styrax is not genotoxic in mammalian bone marrow chromosome aberration test in vivo. In cytotoxicity study, the IC50 values of styrax and its ethanolic extract were found to be 50.22±1.80 and 59.69±11.77 µg/mL, respectively. Among the studied reference standards the major phenolic acids in styrax balsam was found to be p-coumaric acid (2.95 mg/g), while in its ethanolic extract not only p-coumaric acid (11.46 mg/g), but also gallic acid (1.60 mg/g) were found to the main components. Conclusion The findings of the present study provide scientific basis to the safety of styrax from the viewpoint of genotoxicity risk, and in fact, it was found to be beneficial against genotoxicity. © 2016 Elsevier Ireland Lt Daha fazlası Daha az

Gurbuz, I. | Yeşilada, Erdem | Demirci, B. | Sezik, E. | Demirci, F. | Baser, K.H.C.

Article | 2013 | Journal of Ethnopharmacology148 ( 1 ) , pp.332 - 336

https://hdl.handle.net/20.500.11831/806

Süntar, I. | Küpeli Akkol, E. | Keles, H. | Yeşilada, Erdem | Sarker, S.D. | Arroo, R. | Baykal, T.

Article | 2012 | Journal of Ethnopharmacology141 ( 3 ) , pp.1058 - 1070

Ethnopharmacological relevance: In Turkish traditional medicine, the aerial parts of Daphne oleoides Schreber subsp. kurdica (DOK) have been used to treat malaria, rheumatism and for wound healing. The aim was to evaluate the ethnopharmacological usage of the plant using in vivo and in vitro pharmacological experimental models, and to perform bioassay-guided fractionation of the 85% methanolic extract of DOK for the isolation and identification of active wound-healing component(s) and to elucidate possible mechanism of the wound-healing activity. Materials and methods: In vivo wound-healing activity was evaluated by the linear incis . . .ion and the circular excision wound models. Anti-inflammatory and antioxidant activities, which are known to support the wound healing process, were also assessed by the Whittle method and the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assays, respectively. The total phenolic content of the extract and subextracts was estimated to establish any correlation between the phenolic content and the antioxidant activity. The methanolic extract of DOK was subjected to various chromatographic separation techniques leading to the isolation and identification of the active component(s). Furthermore, in vitro hyaluronidase, collagenase and elastase enzymes inhibitory activity assays were conducted on the active components to explore the activity pathways of the remedy. Results: After confirmation of the wound-healing activity, the methanolic extract was subjected to successive solvent partitioning using solvents of increasing polarity creating five subextracts. Each subextract was tested on the same biological activity model and the ethyl acetate (EtOAc) subextract had the highest activity. The EtOAc subextract was subjected to further chromatographic separation for the isolation of components 1, 2 and 3. The structures of these compounds were elucidated as daphnetin (1), demethyldaphnoretin 7-O-glucoside (2) and luteolin-7-O-glucoside (3). Further in vivo testing revealed that luteolin-7-O-glucoside was responsible for the wound-healing activity of the aerial parts. It was also found to exert significant anti-inflammatory, antioxidant, anti-hyaluronidase and anti-collagenase activities. Conclusion: The present study explored the wound-healing potential of Daphne oleoides subsp. kurdica. Through bioassay-guided fractionation and isolation techniques, luteolin-7-O-glucoside was determined as the main active component of the aerial parts. This compound exerts its activity through inhibition of hyaluronidase and collagenase enzymes activity as well as interfering with the inflammatory stage. © 2012 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Akkol, E.K. | Arif, R. | Ergun, F. | Yeşilada, Erdem

Article | 2009 | Journal of Ethnopharmacology122 ( 2 ) , pp.210 - 215

Ethnopharmacological relevance: Several Centaurea species are used to alleviate pain and inflammatory symptoms in rheumatoid arthritis, high fever, and head ache in Turkish folk medicine. Aim of the study: The effectiveness of extracts, fractions and subfractions from dried Centaurea solstitialis L. subsp. solstitialis (CSS) (Asteraceae) roots and aerial parts were studied on mice. Materials and methods: The antinociceptive and antipyretic effects of Centaurea solstitialis L. subsp. solstitialis have been investigated by using p-benzoquinone-induced writhing reflex for antinociceptive activity and Freund's Complete Adjuvant-induced . . .pyrexia model for antipyretic activity assessment in mice. Results: The ethanolic extract from the aerial parts of the plant was shown to possess significant antinociceptive (p < 0.01) and antipyretic activities (p < 0.01). The extract was then submitted to subsequent solvent extractions and chromatographic processes. Through bioassay-guided fractionation and isolation procedures two sesquiterpene lactones, solstitialin A and acetyl solstitialin, were isolated and defined as the active components of CSS. On the other hand, a comparative study was conducted on another species, Centaurea depressa Bieb., which has no similar folkloric utilization. Following the same fractionation chart same compounds were defined as the active ingredients. Conclusion: Results of the present study proved that aerial part of CSS possesses antinociceptive and antipyretic activities supporting the folkloric assertion in Turkish folk medicine. However, these effects seem not limited to CSS, some other Centaurea species, in fact, having no folkloric use might be equally active. © 2009 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Küpeli, E. | Şahin, FikrettinP. | Çaliş, I. | Yeşilada, E. | Ezer, N.

Article | 2007 | Journal of Ethnopharmacology112 ( 2 ) , pp.356 - 360

Acetone extract from aerial parts of Sideritis ozturkii Aytaç & Aksoy and its fractions were investigated for its in vivo anti-inflammatory and antinociceptive activities. For the anti-inflammatory activity assessment, carrageenan-induced hind paw edema and for the antinociceptive activity, p-benzoquinone-induced abdominal constriction tests were used. Acetone extract of the plant and its phenolic fraction were found to possess significant inhibitory activity on these in vivo models in mice. Ozturkoside A (chrysoeriol 7-O-[2'''-O-caffeoyl-6'''-O-acetyl-ß-d-glucopyranosyl-(1 › 2)-ß-d-glucopyranoside]); ozturkoside B (chrysoeriol 7-O- . . .[2'''-O-caffeoyl-ß-d-glucopyranosyl-(1 › 2)-ß-d-glucopyranoside]); and ozturkoside C (chrysoeriol 7-O-[2'''-O-p-coumaroyl-6'''-O-acetyl-ß-d-glucopyranosyl-(1 › 2)-ß-d-glucopyranoside]) were isolated from the active phenolic fraction. The structures of isolated compounds were elucidated by spectroscopic techniques (UV, IR, 1D- and 2D-NMR, MS). Ozturkoside C showed notable antinociceptive and anti-inflammatory activities without inducing any apparent acute toxicity or gastric damage. Although the activity of ozturkosides A and B were found insignificant in statistical analysis, some inhibitory effect was observed. Accordingly, it is suggested that these components in phenolic fraction might possibly share the antinociceptive and anti-inflammatory activities together. © 2007 Daha fazlası Daha az

Kozan, E. | Küpeli, E. | Yeşilada, Erdem

Article | 2006 | Journal of Ethnopharmacology108 ( 2 ) , pp.211 - 216

Ethanolic and aqueous extracts obtained from nine plant species from seven families selected depending on their use in Turkish folk medicine, including Citrillus lanatus (Thunb.) Matsum. (seed), Jasminum fruticans L. (branches), Juniperus drupacea Labill. (fruits), Juniperus nana L. (fruit and leaves), Juniperus oxcycedrus L (fruit and leaves), Mentha longifolia L. (herba), Pinus nigra ssp. pallasiana (Lamb.) Richt. (fruits), Plantago lanceolata L. (leaves), and Zea mays L. (seed) were evaluated for their in vivo anthelmintic activity. Among the plant extracts studied, both ethanolic and aqueous extracts of Jasminum fruticans, Menth . . .a longifolia and Pinus nigra ssp. pallasiana, the aqueous extracts of Zea mays, the ethanolic extracts of Citrillus lanatus, Juniperus drupacea (fruit), Juniperus oxcycedrus and Plantago lanceolata displayed significant anthelmintic activity against pinworms, Syphacia obvelata and Aspiculuris tetraptera, in mice. Rest of the extracts from plants did not show any remarkable anthelmintic activity. The results were considered significant at p < 0.05. © 2006 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Akkol, E.K. | Süntar, I. | Keles, H. | Yeşilada, Erdem

Article | 2011 | Journal of Ethnopharmacology133 ( 3 ) , pp.1027 - 1032

Aim of the study: Female flowers inflorescence of Typha species including Typha domingensis Pers. are used externally for burns and wound healing in Turkish folk medicine. In order to verify the folkloric assertion, the female and male flowers inflorescences were individually submitted to in vivo wound models. Material and methods: Ointment formulations prepared directly either from the male or female flowers inflorescences of Typha domingensis in 5% and 10% concentrations were submitted to activity testing. After that, female flowers inflorescence was further submitted to successive extractions with solvents in increasing polarity; . . . i.e., n-hexane, chloroform, methanol and water and the wound healing activity of each extract was investigated. The linear incision and circular excision wound models were used for the evaluation of the healing potential of the test materials in rats and mice. Tissue sections were also evaluated by histopathological techniques. Results: Remarkable wound healing activity was observed only for the female flowers inflorescence at 5% concentration in ointment base and its methanolic and aqueous extracts. The wound healing effect was found comparable to that of reference ointment Madecassol®. The results of histopathological evaluation supported the outcome of both linear incision and circular excision wound models. Conclusion: The experimental study revealed that the female flowers inflorescence of Typha domingensis displayed notable wound healing activity in mice and rats, at the models tested. © 2010 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az