Gürbüz, I. | Yeşilada, Erdem
Article | 2007 | Journal of Ethnopharmacology112 ( 2 ) , pp.284 - 291
The guaianolide type sesquiterpene lactones chlorojanerin, 13-acetyl solstitialin A and solstitialin A were identified as the anti-ulcerogenic components of the chloroform extract of the aerial parts of Centaurea solstitialis ssp. solstitialis (Asteraceae). In this study, these compounds were investigated by using various in vivo ulcer models in rats and mice. Chlorojanerin was shown to be significantly effective in preventing the induction of lesions by ethanol- (EtOH-) (both oral and subcutaneous administration), indomethacin-, indomethacin plus HCl/EtOH-, NG-nitro-l-arginine methyl ester plus EtOH-, N-ethylmaleimide plus EtOH-, w . . .ater immersion and restraint stress, and serotonin, as well as inhibiting titratable gastric acidity and acid output, and increasing gastric pH, but was ineffective in the prevention of ulcers induced by pyloric ligation, diethyldithiocarbamate, and cysteamine, and had no effect on gastric secretion volume or peptic activity. A mixture of 13-acetyl solstitialin A (95%) and solstitialin A (5%) was found to be significantly effective against EtOH-induced lesions on oral administration but was ineffective when administered subcutaneously. This mixture was also found to be effective in preventing lesions induced by EtOH, indomethacin, indomethacin plus HCl/EtOH, NG-nitro-l-arginine methyl ester plus EtOH, N-ethylmaleimide plus EtOH, water immersion and restraint stress, serotonin and cysteamine, as well as inhibiting titratable gastric acidity and titratable acid output, and gastric pH, but was found ineffective against the pyloric ligation-induced and diethyldithiocarbamate-induced ulcerogenesis models, as well as gastric secretion volume and peptic activity. On the other hand, active compounds did not show any toxic effect on acute toxicity (3 days administration) evaluation tests in mice. © 2007 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az
Demir, A. | Mercanoglu Taban, B. | Aslan, M. | Yeşilada, Erdem | Aykut Aytac, S.
Article | 2009 | Pharmaceutical Biology47 ( 4 ) , pp.289 - 297
The objective of the present study was to investigate the in vitro antibacterial activity of the extracts and their fractions obtained from either the flowers or the leaves of Helichrysum plicatum DC. subsp. plicatum (Asteraceae), by studying inhibition of the growth of a dangerous food pathogen, Escherichia coli O157:H7. The antibacterial effects of the ethanol and water extracts of the flowers and leaves were examined first. Subsequently sub-extracts of the flower ethanol extract (FEE), which was found to be the most effective extract, were examined. As a result, the antibacterial effect of FEE was found to be stronger than that o . . .f the chloroform and ethyl acetate sub-extracts. This may due to synergistic activity of several components of the ethanol extract of the flowers. © 2009 Informa UK Ltd Daha fazlası Daha az
Tatli, I.I. | Akdemir, Z.S. | Yeşilada, Erdem | Kupeli, E.
Article | 2008 | Zeitschrift fur Naturforschung - Section C Journal of Biosciences63 ( 03.04.2020 ) , pp.196 - 202
The potential effects of flavonoids, phenylethanoid and neolignan glycosides from the aerial parts of Verbascum salviifolium Boiss. were studied in the p-benzoquinone-induced writhing reflex, for the assessment of the antinociceptive activity, and in carrageenan- and PGE1-induced hind paw edema and 12-O-tetradecanoyl-13-acetate (TPA)-induced ear edema models in mice, for the assessment of the anti-inflammatory activity. Through bioassay-guided fractionation and isolation procedures ten compounds from the aqueous extract of the plant, luteolin 7-O-glucoside (1), luteolin 3'-O-glucoside (2), apigenin 7-O-glucoside (3), chrysoeriol 7-O . . .-glucoside (4),ß-hydroxyacteoside (5), martynoside (6), forsythoside B (7), angoroside A (8), dehydrodiconiferyl alcohol-9'-O-,ß-D-glucopyranoside (9) and dehydrodiconiferyl alco-hol-9-O-ß-D-glucopyranoside (10), were isolated and their structures were elucidated by spectral techniques. Results have shown that 1, 2, 3 and 5 significantly inhibited carrageenan-induced paw edema at a 200 mg/kg dose, while 1, 2 and 5 also displayed anti-inflammatory activity against the PGE1-induced hind paw edema model. However, all the compounds showed no effect in the TPA-induced ear edema model. The compounds 1 and 2 also exhibited significant antinociceptive activity. © 2008, Verlag der Zeitschrift für Naturforschung. All rights reserved Daha fazlası Daha az
Bardakci, H. | Demirci, B. | Yeşilada, Erdem | Kirmizibekmez, H. | Baser, K.H.C.
Article | 2012 | Records of Natural Products6 ( 1 ) , pp.89 - 92
The subterranean parts of Valeriana alliariifolia Adams were subjected to hydrodistillation and trace amount of essential oil was obtained. The chemical composition of the oil was identified by using capillary Gas Chromatography (GC) and GC/MS simultaneously. In total 68 constituents were identified, representing 87.6% of the total oil. The essential oil was dominated by isovaleric acid (28.6%), which is followed by ?-guaiane (7.2%), ?-humulene (4.7%), hexadecanoic acid (4.3%), valeric acid (3.7%) and humulene epoxide-II (3.6%) as the major components. © 2011Reproduction is free for scientific studies.
Guzelmeric, E. | Ristivojevic, P. | Trifkovic, J. | Dastan, T. | Yilmaz, O. | Cengiz, O. | Yeşilada, Erdem
Article | 2018 | LWT - Food Science and Technology87 , pp.23 - 32
Propolis is a honeycomb product having very diverse chemical composition and possessing a broad spectrum of biological activities. This study comprehensively evaluated the phenolic profile of Turkish propolis by using a high performance thin-layer chromatographic (HPTLC) method in combination with image analysis and pattern recognition technique. Also, botanical origin of each propolis sample was determined by comparison of HPTLC fingerprints of propolis samples with that of plant bud extracts and also by palynological analysis. Moreover, HPTLC coupled with 2,2-diphenyl-1-picrylhydrazyl (DPPH') detection technique was used for scree . . .ning of antioxidant activity of each separated compounds directly on the plate. Results of the present study have demonstrated that Turkish propolis could be classified under three main types; i.e. orange (O) (originated from Populus nigra L.), blue (B) (originated from Populus tremula L.) and nonphenolic types. Palynological analysis have shown that dominant pollen grains (>%45) in propolis samples were: Fabaceae, Lamiaceae, Rosaceae, Castanea sativa Mill., Lotus corniculatus L., Salix spp. In addition, HPTLC-DPPH' results showed that O-type of propolis exerted higher antioxidant activity than the other propolis types. Moreover, quercetin, caffeic acid, caffeic acid phenyl ester, pinobanksin and galangin had significant contribution to the antioxidant activity of propolis. © 2017 Elsevier Lt Daha fazlası Daha az
Akaydın, Galip | Özüdoğru, Barış | Kırmızıbekmez, Hasan | Yeşilada, Erdem
Article | 2011 | Biological Diversity and Conservation4 ( 1 ) , pp.67 - 78
Bu çalışma, İstanbul ili Ataşehir ilçe sınırlarında bulunan Kayış dağı ve Yeditepe Üniversitesi 26 Ağustos Yerleşim Alanı’nın florası ile ilgilidir. Yapılan arazi çalışmaları ve literatür taramaları sonucunda alanda, 73 familyadan 262 cins ve bu cinslere ait toplam 476 taksonun yetiştiği belirlenmiştir. En fazla takson içeren familya Asteraceae (65; %13.7)’dir. Bunu Fabaceae (63; %13.2) ve Poaceae (46; %9.7) izlemektedir. Ayrıca en fazla taksona sahip cins Trifolium L. (19; %4.0) olup, bunu Ranunculus L. (11; %2.3), Vicia L. (8; %1.7) ve Lathyrus L. (8; %1.7) izlemektedir. Araştırma alanındaki 175 (%36.76) taksonun fitocoğrafik bölg . . .esi belirlenebilmiştir. Bu taksonların fitocoğrafik bölgeler içindeki dağılımı şu şekildedir: Akdeniz elementi (105; % 22.06), Avrupa-Sibirya elementi (67; %14.07), İran-Turan elementi (3; %0.63). Araştırma alanında 11 adet endemik (%2.31) ve 3 adet nadir takson tespit edilmiş olup bu bitkilerin IUCN’e göre tehlike kategorileri floristik listeye eklenmiştir. Çalışmanın sonuçları inceleme alanının yakın çevresinde yapılmış olan çalışmalarla fitocoğrafik bölge elementleri ve endemizm yönünden karşılaştırılmıştır. Ayrıca Jaccard benzerlik indeksi kullanılarak alanlar takson benzerlikleri yönünden karşılaştırılmıştır. This study is concerned with the flora of Kayışdağı and Yeditepe University 26 Ağustos Campus, which are located in district of Ataşehir, İstanbul. According to our field studies and literature screening, a total number of 476 taxa which consist of 262 genera from 73 families grow in the area. The highest number of taxa belongs to the familiy Asteraceae (65; 13.7%). It is followed by the families Fabaceae (63; 13.2%) and Poaceae (46; 9.7%). Besides, Trifolium L. (19; 4.0%) is the genus with the highest number of taxa which is followed by Ranunculus L. (11; 2.3%), Vicia L. (8; 1.7%) and Lathyrus L. (8; 1.7%). The phytogeographical regions of 175 (36.76%) taxa in the research field could be identified. The distribution of these taxa in the phytogeographical regions are as follows; Mediterranean element (105; 22.06%), Euro-Siberian element (67; 14.07%), Irano-Turanian element (3; 0.63%). 11 numbers (%2.31) of endemic and 3 numbers of rare taxa were identified in the research field. Moreover, the threat categories of these plants according to IUCN were added to the floristic list. The results of our research are compared with the studied neighboring areas in means of phytogeographic element rates and endemism. Also taxa similarity is compared by Jaccard similarity index Daha fazlası Daha az
Celep, E. | Aydin, A. | Yeşilada, Erdem
Article | 2012 | Food and Chemical Toxicology50 ( 9 ) , pp.3329 - 3335
This study was designed in order to investigate in vitro antioxidant potentials of 80% methanolic extracts prepared from three edible fruits, Cornus mas L., Diospyros kaki L., Laurocerasus officinalis Roem. For this purpose, 8 different tests were performed including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radical scavenging tests, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), metal-chelating capacity, determination of total antioxidant capacity, ß-carotene bleaching test in a linoleic acid emulsion system and trolox equivalent antioxidant capacity. In addition, for evaluating t . . .he phenolic profile, total phenolic, flavonoid and proanthocyanidin contents were measured spectrophotometrically. Among the three fruits analyzed, Diospyros kaki L. showed the highest activity in all tests, except ß-carotene bleaching test. Whereas, neither of three fruits showed metal-chelating activity. Also, a good correlation was found between the phenolic content and antioxidant parameters. © 2012 Elsevier Ltd Daha fazlası Daha az
Süntar, I. | Küpeli Akkol, E. | Keles, H. | Yeşilada, Erdem | Sarker, S.D. | Arroo, R. | Baykal, T.
Article | 2012 | Journal of Ethnopharmacology141 ( 3 ) , pp.1058 - 1070
Ethnopharmacological relevance: In Turkish traditional medicine, the aerial parts of Daphne oleoides Schreber subsp. kurdica (DOK) have been used to treat malaria, rheumatism and for wound healing. The aim was to evaluate the ethnopharmacological usage of the plant using in vivo and in vitro pharmacological experimental models, and to perform bioassay-guided fractionation of the 85% methanolic extract of DOK for the isolation and identification of active wound-healing component(s) and to elucidate possible mechanism of the wound-healing activity. Materials and methods: In vivo wound-healing activity was evaluated by the linear incis . . .ion and the circular excision wound models. Anti-inflammatory and antioxidant activities, which are known to support the wound healing process, were also assessed by the Whittle method and the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assays, respectively. The total phenolic content of the extract and subextracts was estimated to establish any correlation between the phenolic content and the antioxidant activity. The methanolic extract of DOK was subjected to various chromatographic separation techniques leading to the isolation and identification of the active component(s). Furthermore, in vitro hyaluronidase, collagenase and elastase enzymes inhibitory activity assays were conducted on the active components to explore the activity pathways of the remedy. Results: After confirmation of the wound-healing activity, the methanolic extract was subjected to successive solvent partitioning using solvents of increasing polarity creating five subextracts. Each subextract was tested on the same biological activity model and the ethyl acetate (EtOAc) subextract had the highest activity. The EtOAc subextract was subjected to further chromatographic separation for the isolation of components 1, 2 and 3. The structures of these compounds were elucidated as daphnetin (1), demethyldaphnoretin 7-O-glucoside (2) and luteolin-7-O-glucoside (3). Further in vivo testing revealed that luteolin-7-O-glucoside was responsible for the wound-healing activity of the aerial parts. It was also found to exert significant anti-inflammatory, antioxidant, anti-hyaluronidase and anti-collagenase activities. Conclusion: The present study explored the wound-healing potential of Daphne oleoides subsp. kurdica. Through bioassay-guided fractionation and isolation techniques, luteolin-7-O-glucoside was determined as the main active component of the aerial parts. This compound exerts its activity through inhibition of hyaluronidase and collagenase enzymes activity as well as interfering with the inflammatory stage. © 2012 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az
Atay, İrem | Kırmızıbekmez, Hasan | Yeşilada, Erdem | Gören, Ahmet Ceyhan
Other | 2015 | Turkish Journal of Chemistry39 ( 1 ) , pp.34 - 41
Abstract: A new nonglycosidic iridoid, sambulin B (1), was isolated from the methanol extract of Sambucus ebulus L. leaves along with a recently reported new nonglycosidic iridoid, 10-O-acetylpatrinoside aglycone (sambulin A) (2); 2 flavonoids, isorhamnetin-3-O-eta-D-glucopyranoside (3) and isorhamnetin-3-O-rutinoside (4); and a mixture of 2 flavonoids (5), quercetin-3-O-eta-D-glucopyranoside and quercetin-3-O-eta-D-galactopyranoside. Their structures were elucidated by 1-D and 2-D nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) experiments.
Celik, H. | Ariburnu, E. | Baymak, M.S. | Yeşilada, Erdem
Article | 2014 | Analytical Methods6 ( 13 ) , pp.4559 - 4566
In this work, the effects of common cooking practices such as boiling, microwaving, steaming, and oven cooking and their influence on the amount and release of glucoraphanin (GCP) and sulforaphane (SFP) in broccoli and red cabbage were investigated using HPLC. These vegetables are approved for their beneficial effects and have preventing effects particularly against colon, lung, breast, and prostate cancers due to their glucosinolate content, therefore, development of an analytical method for determination of their glucosinolate profile is an important step in clinical studies. The HPLC method that is introduced in this study is ful . . .ly validated and proved to be fast and effective. On the other hand, the importance of the methods of cooking these vegetables has been investigated and compared with each other that resulted in detecting SFP in all samples studied except in samples where whole broccoli was directly added into the boiled water. © the Partner Organisations 2014 Daha fazlası Daha az
Trifkovic, J. | Andric, F. | Ristivojevic, P. | Guzelmeric, E. | Yeşilada, Erdem
Article | 2017 | Journal of AOAC International100 ( 4 ) , pp.827 - 839
Honey is a precious natural product that is marketed with a wide range of nutritional and medicinal properties. However, it is also a product subjected to frequent adulteration through mislabeling and mixing with cheaper and lower-quality honeys and various sugar syrups. In that sense, honey authentication regarding its genuine botanical and geographical origins, as well as the detection of any adulteration, is essential in order to protect consumer health and to avoid competition that could create a destabilized market. Various analytical techniques have been developed to detect adulterations in honey, including measuring the ratio . . .s of stable isotopes (mostly 13C/12C) and the use of different spectroscopic, chromatographic, and electrochemical methods. This review aims to provide a cross-section of contemporary analytical methods used for the determination of honey authenticity in order to help the scientific community engaged in the field of honey chemistry make appropriate choices and select the best applications that should lead to improvements in the detection and elimination of fraudulent practices in honey manufacturing Daha fazlası Daha az
Gurbuz, I. | Yeşilada, Erdem | Ito, S.
Article | 2009 | Journal of Ethnopharmacology121 ( 3 ) , pp.360 - 365
Ethnopharmacological relevance: The aerial parts of Eqiusetum palustre L. is used to treat peptic ulcer in Karaman, Turkey. Aim of the study: The present study was undertaken to evaluate the in vivo gastroprotective potential and structure elucidation of the active constituent(s) through bioassay-guided fractionation procedures by using ethanol (EtOH)-induced ulcerogenesis model in rats. Material and methods: The 80% ethanol extract of the aerial parts was subjected to subsequent separation procedures such as solvent-solvent partition and column chromatography through bioassay-guided fractionation techniques to isolate the active an . . .ti-ulcer component(s) by using the EtOH-induced ulcer model in rats. The ulcer index was used to evaluate and to calculate the gastroprotection percentage. Results: The subextracts, n-butanol and remaining H2O, of Equisetum palustre showed potent in vivo gastroprotective activity. Bioassay-guided fractionation has revealed that a flavonol diglucoside, was isolated as an active constituent from the n-BuOH subextract of Equisetum palustre. Structure elucidation by standard spectroscopic techniques (1H NMR, 13C NMR and DEPT, etc.) revealed that this compound as kaempferol-3-O-1¨-ß-d-glucopyranosyl-3-O-1¨'-ß-d-glucopyranoside, which provided remarkable protection at 250 and 500 mg/kg doses (69.5 and 98.5% ulcer inhibition, respectively) against ethanol-induced gastric lesions. Conclusion: Experimental data confirmed the ethnobotanical usage of Equisetum palustre, with scientific evidence, in Turkey. Additionally, kaempferol-3-O-1¨-ß-d-glucopyranosyl-3-O-1¨'-ß-d-glucopyranoside, a known compound, was defined as an anti-ulcerogenic compound in the present work for the first time. © 2008 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az