Korachi, M | Ceran, N | Adaleti, R | Nigdelioglu, A | Sokmen, M

Article | 2013 | INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES17 ( 1 )

Background: Investigational approaches based on genome-wide association studies have proven useful in identifying genetic predictors for many diseases, including susceptibility to chronic hepatitis B and C. In these studies, the majority of genetic variants that have shown a positive association have been identified in genes involved in the immune response. In this study IFN-gamma, IFNGR-1, and IRF-1 genes were analyzed for their role in susceptibility to the development of chronic hepatitis B and chronic hepatitis C in a Turkish population. Methods: Polymorphic genes IRF-1 (-410, -388), IFNGR-1 (-56, -611), and IFN-gamma (+874) wer . . .e analyzed in a total of 400 individuals: 100 chronic hepatitis B patients, 100 hepatitis B carriers, 100 chronic hepatitis C patients, and 100 healthy controls. A single base primer extension assay was used. Correlations between genes and gender, viral load, and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were also investigated. Results: The IRF-1 gene at positions -388 and -410 were observed to be candidate gene markers for susceptibility to the development of chronic hepatitis B and C (p < 0.05). IFN-gamma +874 and IFNGR-1 (-56 and -611) correlated with chronic hepatitis B but not chronic hepatitis C. Correlation of functional genotype with viral load and AST and ALT levels revealed an association of IFN-gamma +874 and IFNGR-1 -611 with chronic hepatitis C and IFN-gamma +874 with viral load and chronic hepatitis B (p < 0.05). Conclusions: Findings suggest that IFN-gamma (+874), IRF-1 (-410, -388), and IFNGR-1 (-56, -611) are candidate gene markers for determining patient susceptibility to the development of chronic hepatitis B and C. (C) 2012 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved Daha fazlası Daha az

P. Kizilirmak | M. Berktas | Y. Uresin | O.B. Yildiz

Article | 2013 | Journal of Clinical Hypertension15 ( 3 ) , pp.193 - 200

Many hypertensive patients require ?2 drugs to achieve blood pressure targets. This study aims to review and analyze the clinical studies conducted with dual or triple combination of angiotensin II receptor blockers (ARBs), calcium channel blockers (CCBs), and diuretics. Medical literature between January 1990 and April 2012 was reviewed systematically and data from eligible studies were abstracted. Data were analyzed using random-effects models. Of the 224 studies screened, 7563 eligible patients from 11 studies were included. Triple combinations of ARBs (olmesartan or valsartan), CCBs (amlodipine), and diuretics (hydrochlorothiazi . . .de) at any dose provided more blood pressure reduction in office and 24-hour ambulatory measurements than any dual combination of these molecules ( Daha fazlası Daha az

A Kumar | IA Ince | A Kati | R Chakraborty

Article | 2013 | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY63 , pp.511 - 515

A Gram-positive-staining, rod-shaped, facultatively oligotrophic bacterial strain, designated MB18(T), was isolated from a water sample collected from the River Mahananda at Siliguri (26 degrees 44' 23.20' N, 88 degrees 25' 22.89' a West-Bengal, India. On the basis of 16S rRNA gene sequence similarity, the closest relative of this strain was Brevibacterium epidermidis NCDO 2286(T) (96% similarity). The DNA G+C content of strain MB18(T) was 64.6 molo/o. Chemotaxonomic data [MK-8(H-2) as the major menaquinone, galactose as the sole cell-wall sugar, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, phosphatidylglycerol . . . and diphosphatidylglycerol as constituents of the polar lipids, anteiso-C-15:0, anteiso-C-17:0 and iso-C-15:0 as the major fatty acids] supported the affiliation of strain MB18T to the genus Brevibacterium. The results of DNA G +C content, 16S rRNA gene sequence analysis and biochemical and physiological analyses allowed genotypic and phenotypic differentiation of strain MB18(T) from its nearest neighbour B. epidermidis. The isolate therefore represents a novel species, for which the name Brevibacterium siliguriense sp. nov. is proposed; the type strain is MB18(T) (=DSM 23676(T)=LMG 25772(T)) Daha fazlası Daha az

A. Kumar | I.A. Ince | A. Kati | R. Chakraborty

Article | 2013 | International Journal of Systematic and Evolutionary Microbiology63 ( PART2 ) , pp.511 - 515

A Gram-positive-staining, rod-shaped, facultatively oligotrophic bacterial strain, designated MB18T, was isolated from a water sample collected from the River Mahananda at Siliguri (26° 44' 23.20' N, 88° 25' 22.89' E), West-Bengal, India. On the basis of 16S rRNA gene sequence similarity, the closest relative of this strain was Brevibacterium epidermidis NCDO 2286T (96% similarity). The DNA G+C content of strain MB18T was 64.6 mol%. Chemotaxonomic data [MK-8(H2) as the major menaquinone, galactose as the sole cell-wall sugar, meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, phosphatidylglycerol and diphosphatidylgl . . .ycerol as constituents of the polar lipids, anteiso-C15: 0, anteiso-C17: 0 and iso-C15: 0 as the major fatty acids] supported the affiliation of strain MB18T to the genus Brevibacterium. The results of DNA G+C content, 16S rRNA gene sequence analysis and biochemical and physiological analyses allowed genotypic and phenotypic differentiation of strain MB18T from its nearest neighbour B. epidermidis. The isolate therefore represents a novel species, for which the name Brevibacterium siliguriense sp. nov. is proposed; the type strain is MB18T (=DSM 23676T=LMG 25772T). © 2013 IUMS Daha fazlası Daha az

Ince, I.A. | Özcan, K. | Vlak, J.M. | van Oers, M.M.

Article | 2013 | Journal of General Virology94 ( PART11 ) , pp.187 - 192

The temporal expression of the 54 Chilo iridescent virus (CIV) virion protein genes was investigated by combining drug treatments that inhibit protein or DNA synthesis and an RT-PCR strategy particularly suitable for non-polyadenylated mRNAs. This method generates a uniform 3' terminus by ligation of a 5'-phosphorylated oligonucleotide to the 3' end of the transcript that is recognized by a complementary primer during RT-PCR. This analysis showed that CIV virion proteins are encoded by genes in all three predetermined temporal classes: 23 immediate-early, 11 delayed-early and seven late virion gene transcripts were identified and as . . .signed to ORFs. Early transcription of many virion protein genes supports the notion that virion proteins may also play essential roles in the initial stages of infection. In addition, some of the early gene products present in the virion may reflect the intracellular path that the virus follows during infection. © 2013 SGM Daha fazlası Daha az

Tuncer, I | Delilbasi, C | Deniz, E | Soluk-Tekkesin, M | Olgac, V | Sencift, K

Article | 2017 | JOURNAL OF ISTANBUL UNIVERSITY FACULTY OF DENTISTRY51 ( 1 ) , pp.8 - 14

Purpose: Bisphosphonates are commonly used drugs in pediatric patients in the treatment of osteoporotic diseases and various types of cancers. The purpose of this study was to evaluate the effects of pamidronate administration on mandibular growth and tooth eruption in new born rats. Materials and Methods: Forty Sprague Dawley rats were included in the study and divided into four groups as; 14th day pamidronate group, 30th day pamidronate group, 14th day control group and 30th day control group. Pamidronate groups were daily injected with 1.25 mu g/g pamidronate disodium subcutaneously whereas control groups were injected with steri . . .le saline. Eruption levels of lower incisor and molar teeth were assessed macroscopically. Mandibular growth was assessed by measuring reference points in cone beam tomography. Histological and histomorphometric examinations were performed under light microscope to evaluate tooth morphology and number of osteoclasts. Results: Retardation in mandibular growth, decrease in number of osteoclasts, delay in tooth eruption, degeneration in both tooth morphology and structure were observed in the pamidronate groups compared to control groups. Conclusion: Pamidronate administration during growth and development stage may adversely affect tooth eruption and mandibular growth in new born rats Daha fazlası Daha az

Gümral, D. | Fogolari, F. | Corazza, A. | Viglino, P. | Giorgetti, S. | Stoppini, M. | Esposito, G.

Article | 2013 | Magnetic Resonance in Chemistry51 ( 12 ) , pp.795 - 807

The amyloid pathology associated with long-term haemodialysis is due to the deposition of ß2-microglobulin, the non-polymorphic light chain of class I major histocompatibility complex, that accumulates at bone joints into amyloid fibrils. Several lines of evidence show the relevance of the tryptophan residue at position 60 for the fibrillogenic transition of the protein. A comparative 15N NMR relaxation analysis is presented for wild-type human ß2-microglobulin and W60G ß2- microglobulin, i.e. the mutant with a glycyne replacing the natural tryptophan residue at position 60. The experimental data, collected at 11.4 T and 310 K, were . . . analyzed by means of the reduced spectral density approach. Molecular dynamics (MD) simulations and corresponding thermodynamic integration, together with hydrodynamic calculations were performed to support data interpretation. The analysis results for the mutant protein are consistent with a reduced aggregation with respect to the wild-type counterpart, as a consequence of an increased conformational rigidity probed by either NMR relaxation and MD simulations. Although dynamics in solution is other than fibrillar competence, the assessed properties of the mutant protein can be related with its reduced ability of forming fibrils when seeded in 20% trifluoroethanol. Copyright © 2013 John Wiley & Sons, Ltd Daha fazlası Daha az

Basak, T. | Uzun, S.

Article | 2013 | Clinical Nurse Specialist27 ( 4 ) , pp.194 - 197

OBJECTIVE: The objective of this study was to compare the rates of urine contamination and the frequencies of minor adverse events between the vacuum tube and injector techniques methods for the collection of sterile urine specimens. DESIGN: This study was a cross-sectional study. SAMPLE: The sample population was composed of patients who underwent a urodynamics procedure. SETTING: This study was performed at the urodynamics unit of a rehabilitation and care center in Turkey. METHODS: Sterile urine specimens (n = 576) were collected from 144 patients using the vacuum tube technique and the injector technique; the rates of urine cont . . .amination and minor adverse events were evaluated. ? Statistics and Fisher exact tests were used to assess the contamination rates and frequencies of minor adverse events during the collection of sterile urine specimens. RESULTS: The contamination rate of urine specimens collected using an injector was 0.9% (P > .05). The rate of minor adverse events during specimen collection was lower when using the vacuum technique than when using an injector and sterile urine container (2 vs 36 events, P < .05). CONCLUSION: The vacuum tube technique of urine specimen collection was not superior to the injector technique in regard to contamination. The use of the vacuum tube technique for the collection of sterile urine specimens can prevent minor adverse events related to spillage/leakage of urine. IMPLICATIONS: More research from multiple settings and work environments is needed to strengthen the evidence base of vacuum tube technique of sterile urine specimen collection. Copyright © 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins Daha fazlası Daha az

Yilmaz, D. | Yüksel, D. | Şenbil, N. | Eminzade, S. | Kilinç, K. | Anlar, B. | Gürer, Y.

Article | 2009 | Pediatric Neurology41 ( 3 ) , pp.179 - 182

Oxidative damage plays a role in neurodegenerative diseases. Levels of cerebrospinal fluid nitrite and nitrate levels (oxidation products that provide an indirect estimation of nitric oxide) were investigated in relation to clinical and laboratory features in subacute sclerosing panencephalitis (n = 47) and age-matched control (n = 43) groups. Significantly decreased levels of nitrite (median, 4.91 µmol/L) and nitrate (median, 6.14 µmol/L) were found in the patients. Nitrite and nitrate levels did not correlate with clinical or laboratory findings, except for presence of myoclonus. Cerebrospinal fluid nitrite levels of subacute scle . . .rosing panencephalitis patients without myoclonic jerks were significantly higher than in those with myoclonus (median, 15.63 vs 4.34 µmol/L, respectively). The higher levels of nitrite in these patients can be explained by short disease duration and early stages of disease. Nitrate levels in subacute sclerosing panencephalitis patients with myoclonus (median, 9.26 µmol/L) were higher than in those without myoclonus (median, 4.25 µmol/L). Microbleeding resulting in conversion of nitrite to nitrate and increased production of superoxide can be suggested as possible mechanisms underlying these findings. © 2009 Elsevier Inc. All rights reserved Daha fazlası Daha az

Ilguy, M | Ilguy, D | Fisekcioglu, E | Oktay, I

Article | 2014 | JOURNAL OF DENTAL EDUCATION78 ( 11 ) , pp.1521 - 1527

The aim of this study was to compare the impact of case-based learning (CBL) and lecture-based learning (LBL) on fourth-year dental students' clinical decision making by using the Structure of Observed Learning Outcome (SOLO) taxonomy. Participants in the study were fourth-year dental students (n=55) in academic year 2012-13 taught in a large-group LBL context and fourth-year dental students (n=54) in academic year 2013-14 taught with the CBL methodology; both took place in the oral diseases course at Yeditepe University Faculty of Dentistry, Istanbul, Turkey. All eligible students participated, for a 100 percent response rate. A re . . .al case was presented to the students in both groups to assess their clinical decision making on the topic of oral diseases. Their performance was evaluated with the SOLO taxonomy. Student t-test was used for statistical evaluation, and significance was set at the Daha fazlası Daha az

Inci, G. | Sorgüven, E.

Article | 2012 | ASAIO Journal58 ( 4 ) , pp.373 - 381

Left ventricular assist devices (LVADs), which pump blood from the left ventricle to the aorta are an important therapy option for patients with end-stage cardiovascular diseases. Recent publications show that even with optimized LVADs fatal complications can occur because of the blood deformations around the inflow cannula or through the LVAD outlet graft-aorta anastomosis. This study investigates the effects of the anastomosis geometry on the flow through the aorta, on the pressure and wall shear stress (WSS) distributions on the aortic wall and on the total entropy generation in the anastomosis region. Anastomosis geometry is def . . .ined with two angles, one on the coronal plane and the other on the transversal plane. Turbulent flow simulations are performed for each geometry. Results indicate that 3% to 5% of the work given by the LVAD is dissipated because of the viscous losses in the anastomosis region. The entropy generation, as well as the maximum WSS, increases as the inclination angle decreases. Some portion of the blood streaming out of the LVAD conduit flows toward the aortic valve; therefore the reverse-flow region extends up to the aortic valve in some cases, which may be one of the causes of aortic-valve dysfunction. Results of this study provide insight on the importance of the anastomosis geometry on the hemodynamics in the aorta and downstream the aortic valve, stresses on the aortic wall, and viscous losses. Copyright © 2012 by the American Society for Artificial Internal Organs Daha fazlası Daha az

Turgut, H.B. | Peker, T. | Karaköse, M. | Anil, A. | Gülekon, N. | Demirtaş, K.

Article | 2006 | Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia35 ( 6 ) , pp.357 - 360

The best method to evaluate the pathogenesis of diaphragmatic disorders is to demonstrate the distribution pattern of the phrenic nerve in the diaphragm. For this purpose the branching pattern and the microanatomic features of the phrenic nerve were observed in six rabbits. All diaphragms were stained by using Sihler's stain method. The phrenic nerve divided into three to four branches when entering the diaphragm. These branches were classified as sternal, anterolateral, posterolateral and crural. The crural branches were the thickest whereas the anterolateral branches were the thinnest. Knowledge about the distribution pattern of t . . .he phrenic nerve may be important in surgical approach to the diaphragm. © 2006 Blackwell Verlag Daha fazlası Daha az