Türkekul, I. | Yilmaz, N. | Şahin, Fikrettin | Bayrak, Ö.F.

Article | 2010 | Asian Journal of Chemistry22 ( 2 ) , pp.1479 - 1486

The aim of this study is to determine the amount and composition of fatty acids in 6 edible mushroom species belonging to the different families grown in the middle Black sea region (Tokat, Amasya, Ordu provinces). The amount and composition of fatty acids in dried mushrooms samples were detected with a gas chromatography. The fatty acids were determined using the fatty acids methyl-esters standards. Fatty acids with 10-20 numbers were ocurred in mushrooms samples. Also, fatty acids with a single carbon and single double bound were found. Linoleic acid (18:2 w6c) occurred more than 50 % in most of the samples studied. Fatty acit ana . . .lysis of the mushroom in the present study showed that unsaturated fatty acids were higher than the unsaturated fatty acids Daha fazlası Daha az

Agar, G. | Adiguzel, A. | Baris, O. | Gulluce, M. | Şahin, Fikrettin | Sengul, M.

Article | 2010 | Bangladesh Journal of Botany39 ( 1 ) , pp.1 - 7

Fatty acid (FA) and RAPD profiles were used to examine phenotypic and genotypic relationships, respectively among Astragalus atrocarpus, A. campylosema subsp. campylosema, A. fumosus, A. galegiformes, A. lineatus var. jildisianus and A. trichostigma. All the taxa were separated based on the presence and composition of 33 different FAs. A set of nine FAs (14 : 0, 16 : 0, 18 : 0, 18 N alcohol, 17 : 1 iso G, 18: 1 : ?8c, C18 : 1 : ?9t alcohol, 18:2: ?6c, 19:1: ?6c) were found in all Astragalus taxa. Six of the ten decamer primers examined were selected to explore the genetic variation among the taxa tested. The RAPD results suggested t . . .hat A. fumosus, A. lineatus and A. trichostigma are closely related and completely different from the rest of the taxa. Four genetically distinct groups were found among the species. High genetic variations were present among species growing wildly in eastern Anatolia region of Turkey, which may imply differences in their origin. It appears that both RAPD and FA analyses are useful for differentiation of Astragalus species Daha fazlası Daha az

Kesmen, Z. | Yetiman, A.E. | Şahin, Fikrettin | Yetim, H.

Article | 2012 | Journal of Food Science77 ( 2 ) , pp.1 - 7

In this study, TaqMan-based real-time Polymerase Chain Reaction (PCR) techniques were developed for the detection of chicken and turkey meat in raw and heat-treated meat mixtures. Primers and TaqMan probe sets were designed to amplify 86 bp and 136 bp fragments for the chicken and turkey species, respectively, on the mitochondrial NADH dehydrogenase subunit 2 gene. In the results, it was possible to detect each species at the level of 0.1 pg template DNA with the TaqMan probe technique without any cross-reactivity with nontarget species (bovine, ovine, donkey, pork, and horse) while the detection level was 1 pg template DNA using co . . .nventional PCR. The TaqMan probe assays used in this study allowed the detection of as little as 0.001% level of both species in the experimental meat mixtures, prepared by mixing chicken and turkey meat with beef at different levels (0.001% to 10%). In conclusion, TaqMan probe assays developed in this research are promising tools in the specific identification and sensitive quantification of meat species even in the case of heat-treated meat products, and suitable for a rapid, automated, and routine analysis. © 2012 Institute of Food Technologists ® Daha fazlası Daha az