Yildirim, G. | Attar, Rukset | Ficicioglu, C. | Karateke, A. | Ozkan, F. | Yesildaglar, N.

Article | 2011 | Archives of Gynecology and Obstetrics283 ( 6 ) , pp.1297 - 1302

Objective: To determine the effects of etanercept (anti-TNF-?) on surgically induced endometriosis in a rat model. Materials and methods: This is a prospective, randomized, controlled, experimental study that was carried out at the Experimental Research Center of Yeditepe University (YUDETAM). Thirty female nonpregnant, nulligravid Wistar-Hannover albino rats were used. The summary of the technique: surgical induction of endometriosis, administration of estrogen for 2 weeks, and laparotomy; administration of etanercept for 2 weeks following the induction of endometriosis and laparotomy; administration of estrogen for 2 weeks and nec . . .ropsy. The volume and histopathological scores of the endometriotic foci were evaluated. Results: One-hundred twenty uterine horns were implanted in 30 rats. Endometriosis was completely formatted in 112 lesions (93.3%). No rats were lost. In the etanercept group, the lesions' volumes were 83.9 ± 13.1, 47.2 ± 8.4, and 96.7 ± 34.8 mm3 at the end of the second week (pretreatment stage), at the end of the fourth week (post-treatment stage), and at the end of the sixth week, respectively (P = 0.007). Histopathologic scores were 2.3 ± 0.2, 1.7 ± 0.2, and 1.9 ± 0.1, respectively (P = 0.08). The changes in the other groups were not statistically significant. Conclusions: Etanercept, a fusion protein consisting of human recombinant soluble TNF receptor-2, neutralizes TNF activity. Anti-TNF therapy could be a new non-hormonal therapeutic option for the treatment of endometriosis in humans. © 2010 Springer-Verlag Daha fazlası Daha az

Cakmakoglu, B.A. | Attar, Rukset | Kahraman, O.T. | Dalan, A.B. | Iyibozkurt, A.C. | Karateke, A. | Attar, E.

Article | 2011 | Molecular Biology Reports38 ( 5 ) , pp.3481 - 3486

In this study, we aimed to investigate a possible association of the COX-2 polymorphisms (-765G›C and -1195A›G) and with the risk of developing epithelial ovarian carcinoma (EOC). COX-2 gene polymorphisms was investigated in 111 healthy women and 57 patients with EOC. Individuals who had -765 CG, -1195 AA genotype, and -765 C allele had increased risk for ovarian carcinoma (P

Attar, Rukset | Yildirim, G. | Kumbak, B. | Ficicioglu, C. | Demirbag, S. | Yesildaglar, N.

Article | 2011 | Journal of Obstetrics and Gynaecology Research37 ( 2 ) , pp.125 - 131

Aim: To evaluate the efficacy of hyaluronate/carboxymethylcellulose (HA/CMC) membrane and melatonin separately and in combination in reducing adhesion reformation following adhesiolysis of surgically induced adhesions in a rat uterine horn adhesion model. Methods: A randomized, prospective study was carried out in a university animal laboratory. Ninety-eight female Sprague-Dawley albino rats were operated on. Following infliction of standard lesions, all the animals underwent second operations after one week. In all the animals, there were dense and vascular adhesions only between the uterine horns. These adhesions were lysed. Follo . . .wing the completion of adhesiolysis, the animals were randomized before closure of the abdomen to one of four groups (melatonin, HA/CMC membrane, combination of melatonin and HA/CMC membrane, control group). Seven days after the second surgery, the third operations were carried out and adhesions were scored. The main outcome measures were type, tenacity, and extent of adhesions. Total adhesion scores were determined. Results: Adhesion scores in the melatonin and HA/CMC membrane groups were similar, and significantly lower than those in the control group (P < 0.001). Adhesion scores in the combination group were lower than those in the other three groups (P < 0.001). Conclusion: Melatonin and HA/CMC membrane are both effective separately in preventing adhesion reformation following adhesiolysis, but in combination they are signiicantly more beneicial. © 2010 Japan Society of Obstetrics and Gynecology Daha fazlası Daha az