Bostanci, M.S. | Bakacak, M. | Yildirim, O.K. | Yildirim, G. | Attar, Rukset | Ozkan, F. | Tolun, F.I.

Article | 2016 | Clinical and Experimental Obstetrics and Gynecology43 ( 4 ) , pp.529 - 533

Purpose of investigation: To evaluate the preventive and reducing effect of aloe vera gel on surgically-induced endometrial foci in rats. Materials and Methods: Twenty-four reproductive aged female non-pregnant, nulligravid Sprague-Dawley albino rats were used. The rats were randomly divided to three groups (Group 1: control. Group 2: Aloe vera endometriosis formation, and Group 3: Aloe vera endometriosis treatment). A peritoneal lavage using one-ml saline was taken at all the operations for determination of superoxide disinutase (SOD), malondialdehyde (MDA), and catalase (CAT). Forty-eight horns were implanted in 24 rats. Results: . . .All the implants were properly formed after implantation. In Group 3, before aloe vera application, the sum of the volumes was 87.2 ± 20.4 mm' and after treatment the volumes dropped to 28.9 ± 14.9 mm' (p = 0.01). As evaluation of aloe vera on the formation of endometriosis in the second operation in Group 2, the sum of the volumes was 2.9*1.4 mm3 and in Group 1, 118.9 ± 20.0 mm5 (/> = 0.001). Likewise, similar changes were observed in the histopatological scores. Conclusion: The application of aloe vera was seen to raise antioxidant levels in the peritoneal fluid and to reduce oxidative stress markers. Aloe vera is effective in the inhibition of formation and regression of endometriotic lesions Daha fazlası Daha az

Yesildaglar, N. | Yildirim, G. | Yildirim, O.K. | Attar, Rukset | Ozkan, F. | Akkaya, H. | Yılmaz, B.

Article | 2016 | Clinical and Experimental Obstetrics and Gynecology43 ( 1 ) , pp.25 - 30

Objective: To evaluate the effects of melatonin on endometriotic lesions induced by implanting human endometriotic cells in SCID mice. Materials and Methods: Prospective, randomized, controlled, experimental study. Experimental Research Center of Yeditepe University (YUDETAM). Thirty female, non-pregnant, nulligravid severe combined immunodeficient (SCID) mice. Endometriotic cells collected from patients with endometriosis were implanted subcutaneously in 30 SCID mice. These mice were randomized into two study groups: in the first group, mice were administered melatonin (20 mg/kg/day) following induction of endometriosis for four we . . .eks; in the second group, nothing was administered. All the mice were given a high dose of exogenous estradiol (50 µg/kg/d, twice weekly). Four weeks after inoculation, necropsies were performed and endometriotic lesions were collected. All the lesions were evaluated histopathologically and the levels of SOD and MDAwere assessed in the lesions. Results: Successful implantation was observed in the 28 mice that survived. Mean MDA level was 5.0±1.7 and 8.8±2.6 in the melatonin and control groups, respectively (p = 0.01); mean SOD level was 1.1 ± 0.1 and 1.0 ± 0.1 in the melatonin and control groups, respectively (p = 0.49). Mean histopathological score was lower in the melatonin group (p = 0.04). Conclusions: Melatonin was effective in the treatment of experimental endometriosis induced in SCID mice Daha fazlası Daha az