Cetinkaya, N. | Attar, Rukset | Yildirim, G. | Ficicioglu, C. | Ozkan, F. | Yılmaz, B. | Yesildaglar, N.

Article | 2015 | Archives of Gynecology and Obstetrics291 ( 3 ) , pp.591 - 598

Aims: To determine the effects of different doses of melatonin treatment on endometrial implants, the activity of antioxidant enzyme superoxide dismutase (SOD), the angiogenesis factor, the vascular endothelial growth factor (VEGF) and the waste metabolite product of lipid peroxidation malondialdehyde (MDA) in an oophorectomized rat endometriosis model.Methods: Thirty-two, female, non-pregnant, nulligravid Sprague–Dawley, albino rats were used in this prospective, randomized, controlled and experimental study. Endometriosis was surgically induced in oophorectomized rats, and estradiol treatment was started after the first operation . . .and continued till the end of the study. Second look, third look and necropsy operations were performed in the 2nd, 4th and 6th weeks. Mean volumes, histological scores and biochemical parameters were evaluated throughout the study.Results: The mean volumes of endometriotic foci were 98.8 mm3 ± 17.2 vs. 108.2 mm3 ± 17.5, 54.1 mm3 ± 15.6 vs. 25.8 mm3 ± 3.6, 42.8 mm3 ± 10.5 vs. 32.7 mm3 ± 6.0 and histopathological scores were 2.2 ± 0.2 vs. 1.7 ± 0.1, 2.6 ± 0.2 vs. 2.2 ± 0.2, 2.6 ± 0.1 vs. 2.7 ± 0.2 in the 10 vs. 20-mg/kg/day melatonin group at the end of the second, fourth and sixth weeks, respectively. When the groups were compared, no significant differences were seen in the histopathologic scores, SOD and VEGF levels between the groups. However, the endometriotic foci volumes were significantly decreased in both melatonin treatment groups with respect to the control group at the end of the fourth and sixth weeks. Moreover, the mean MDA levels were significantly lower in the control group than in the 10-mg/kg/day melatonin group at the end of the fourth and sixth weeks.Conclusion: Melatonin treatment resulted in the regression of endometriotic lesions in oophorectomized rats. Higher doses of melatonin treatment might be more effective in the regression of implants and improvement of histologic scores as well as in the precise evaluation of SOD, MDA and VEGF distributions in the rat experimental models. © 2014, Springer-Verlag Berlin Heidelberg Daha fazlası Daha az

Narin, R. | Attar, Rukset | Narin, M.A. | Koyuncu, D. | Yencilek, E.

Article | 2014 | Archives of Gynecology and Obstetrics290 ( 5 ) , pp.913 - 917

Results: After TOT procedure, the mean value of IIQ-7 and UDI-6 questionnaires was lower than the mean scores that measured before treatment (p 

Bostancı, M.S. | Bakacak, M. | İnanc, F. | Yaylalı, A. | Serin, S. | Attar, Rukset | Yildirim, G.

Article | 2016 | Archives of Gynecology and Obstetrics293 ( 4 ) , pp.789 - 795

Objective: This experimental study was designed to evaluate the effect of granulocyte colony-stimulating factor (G-CSF) in ovarian ischemia and ischemia/reperfusion (I/R) injury. Study design: Forty-eight female adult Sprague–Dawley albino rats were divided into six groups as Group 1: sham, Group 2: torsion, Group 3: detorsion, Group 4: sham + G-CSF, Group 5: torsion + G-CSF, and Group 6: detorsion + G-CSF. Except for Groups 1 and 4, all groups underwent adnexal torsion bilaterally for 3 h. Adnexal detorsion was applied to Groups 3 and 6 for 3 h after a 3-h torsion period. The intraperitoneal injection of G-CSF (100 IU/kg) was admin . . .istered 30 min previously in Group 4, 5 and 6. At the end of the study process the animals were euthanized and their ovaries were removed for histopathological and biochemical analysis. Total oxidant status (TOS), total antioxidative status and oxidative stress index (OSI) concentrations were determined and compared. Histopathological examination of ovaries was performed for the presence of interstitial edema, congestion, hemorrhage and loss of cohesion to determine tissue damage. Results: In Group 3, 4, 5 and 6, TOS, OSI and total histopathological scores of ovarian tissue were higher than in the sham group (p Daha fazlası Daha az