Suntar, I | Akkol, EK | Keles, H | Yesilada, E | Sarker, SD | Arroo, R | Baykal, T

Article | 2012 | JOURNAL OF ETHNOPHARMACOLOGY141 ( 3 ) , pp.1058 - 1070

Ethnopharmacological relevance: In Turkish traditional medicine, the aerial parts of Daphne oleoides Schreber subsp. kurdica (DOK) have been used to treat malaria, rheumatism and for wound healing. The aim was to evaluate the ethnopharmacological usage of the plant using in vivo and in vitro pharmacological experimental models, and to perform bioassay-guided fractionation of the 85% methanolic extract of DOK for the isolation and identification of active wound-healing component(s) and to elucidate possible mechanism of the wound-healing activity. Materials and methods: In vivo wound-healing activity was evaluated by the linear incis . . .ion and the circular excision wound models. Anti-inflammatory and antioxidant activities, which are known to support the wound healing process, were also assessed by the Whittle method and the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assays, respectively. The total phenolic content of the extract and subextracts was estimated to establish any correlation between the phenolic content and the antioxidant activity. The methanolic extract of DOK was subjected to various chromatographic separation techniques leading to the isolation and identification of the active component(s). Furthermore, in vitro hyaluronidase, collagenase and elastase enzymes inhibitory activity assays were conducted on the active components to explore the activity pathways of the remedy. Results: After confirmation of the wound-healing activity, the methanolic extract was subjected to successive solvent partitioning using solvents of increasing polarity creating five subextracts. Each subextract was tested on the same biological activity model and the ethyl acetate (EtOAc) subextract had the highest activity. The EtOAc subextract was subjected to further chromatographic separation for the isolation of components 1,2 and 3. The structures of these compounds were elucidated as daphnetin (1), demethyldaphnoretin 7-O-glucoside (2) and luteolin-7-O-glucoside (3). Further in vivo testing revealed that luteolin-7-O-glucoside was responsible for the wound-healing activity of the aerial parts. It was also found to exert significant anti-inflammatory, antioxidant, anti-hyaluronidase and anti-collagenase activities. Conclusion: The present study explored the wound-healing potential of Daphne oleoides subsp. kurdica. Through bioassay-guided fractionation and isolation techniques, luteolin-7-O-glucoside was determined as the main active component of the aerial parts. This compound exerts its activity through inhibition of hyaluronidase and collagenase enzymes activity as well as interfering with the inflammatory stage. (C) 2012 Elsevier Ireland Ltd. All rights reserved Daha fazlası Daha az

Kirmizibekmez, H | Atay, I | Kaiser, M | Yesilada, E | Tasdemir, D

Conference Object | 2009 | PLANTA MEDICA75 ( 9 ) , pp.904 - 905

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Tosun, A | Akkol, EK | Yesilada, E

Article | 2009 | ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES64 ( 01.02.2020 ) , pp.56 - 62

n-Hexane and ethyl acetate extracts as well as coumarin derivatives obtained from the n-hexane extract of the aerial parts of Seseli gummiferum Pall. ex Sm. subsp. corymbosum (Boiss. & Heldr.) P.H. Davis (Apiaceae) were evaluated in vivo for their anti-inflammatory and antinociceptive activities. The n-hexane and ethyl acetate extracts of the species were shown to possess significant inhibitory activity against the carrageenan-induced hind paw edema and p-benzoquinone-induced writhing models in mice. Among the isolated coumarin derivatives; (-)-(3'S,4'S)-3'-acetoxy-4'-isovaleryloxy-3',4'-dihydroseselin (1), (-)-(3'S14 S)-3'-acetoxy- . . .4'-angeloyloxy-3'.4'-dihydroseselin (2),(+)-(3'S,4'S)-3'-hydroxy-4'-angeloyloxy-3',4'dihydroseselin (D-laserpitin) (3), (-)-(3'S,4'S)-3'-angeloyloxy-4'-hydroxy-3',4'-dihydroseselin (4), and osthole (5), only the 3'-acetoxy derivatives 1 and 2 were found to possess potent antinociceptive and anti-inflammatory activities, per os, without inducing any apparent acute toxicity as well as gastric damage, while all other compounds and extracts were found to be ineffective in the TPA-induced mouse ear edema model assay Daha fazlası Daha az

Kirmizibekmez, H | Demirci, B | Yesilada, E | Baser, KHC | Demirci, F

Article | 2009 | NATURAL PRODUCT COMMUNICATIONS4 ( 7 ) , pp.1001 - 1006

The chemical compositions of the essential oils obtained by hydrodistillation from file dried leaves and flowers of Lavandula stoechas L. ssp. stoechas were separately identified by GC-FID and GC-MS analyses. The main components were alpha-fenchone (41.9 +/- 1.2%), 1,8-cincole (15.6 +/- 0.8%), camphor (12.1 +/- 0.5%), and viridiflorol (4.1 +/- 0.4%) in the leaves; and alpha-fenchone (39.2 +/- 0.9%), myrtenyl acetate (9.5 +/- 0.4%), alpha-pinene (6.1 +/- 0.09%), camphor (5.9 +/- 0.05%) and 1,8-cincole (3.8 +/- 0.1%) in the flowers. Overall, 55 and 66 Constituents were identified in the leaf and flower essential oils representing more . . . than 90% and 94% of the total, respectively. In addition, the essential oils were evaluated tor their antibacterial and anticandidal activities by broth rnicrodilution. The flower essential oil was found to be relatively more active than the leaf oil towards the tested pathogenic microorganisms. Methicillin-resistant Staphylococcus aureus was more susceptible to the flower oil (MIC= 31.2 mu g/mL). The oils, evaluated for their free radical Scavenging activity using a TLC-DPPH assay, were inactive at a concentration of 2 mg/mL Daha fazlası Daha az

Atay, I | Kirmizibekmez, H | Goren, AC | Yesilada, E

Article | 2015 | TURKISH JOURNAL OF CHEMISTRY39 ( 1 ) , pp.34 - 41

A new nonglycosidic iridoid, sambulin B (1), was isolated from the methanol extract of Sambucus ebulus L. leaves along with a recently reported new nonglycosidic iridoid, 10-O-acetylpatrinoside aglycone (sambulin A) (2); 2 flavonoids, isorhamnetin-3-O-beta-D-glucopyranoside (3) and isorhamnetin-3-O-rutinoside (4); and a mixture of 2 flavonoids (5), quercetin-3-O-beta-D-glucopyranoside and quercetin-3-O-beta-D-galactopyranoside. Their structures were elucidated by 1-D and 2-D nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) experiments.

Yesilada, E

Article | 2015 | ETHNOPHARMACOLOGY , pp.356 - 362

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Koksal, M | Gokhan, N | Kupell, E | Yesilada, E | Erdogan, H

Article | 2005 | ARCHIV DER PHARMAZIE338 ( 02.03.2020 ) , pp.117 - 125

The synthesis of a novel series of Mannich bases of 5-/6-acyl-5-methyl-2-benzoxazolinones has been described. The structures attributed to compounds 2a, 3a, 4a, 4b, 9a, 9b, 5a-5g, 6a-6g, 10a, 10g, 11a, 11g have been elucidated using IR and H-1 NMR spectroscopic techniques besides elemental analysis. The compounds have been evaluated for their in vivo analgesic and antiinflammatory activities using the p-benzoquinone-induced writhing test and the carrageenan hind paw oedema test in mice, respectively. In addition, the ulcerogenic effects of the compounds were determined. Among the tested derivatives most promising results were obtain . . .ed for the compounds bearing a 6-(4-chlorobenzoyl) at C-6 position and 2-/4-fluorophenyl at C-3 position of 2-benzoxazolinone ring (11c, 11d) Daha fazlası Daha az

Gurbuz, I | Demirci, B | Franz, G | Baser, KHC | Yesilada, E | Demirci, F

Article | 2013 | TURKISH JOURNAL OF BIOLOGY37 ( 1 ) , pp.114 - 121

Aerial parts of Daphne pontica were collected from Ilgaz-cankiri, and D. oleoides subsp. oleoides was collected from 2 different localities (Ayranci-Karaman and Ilgaz-Cankiri) in Turkey. The samples were subjected to hydrodistillation and microdistillation. The resulting volatile samples were analyzed both by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), respectively. The main components for D. pontica were identified as hexahydrofarnesyl acetone (8.6%), carvacrol (8.5%), dihydroedulane 11 (4.7%), (E)-geranyl acetone (4.6%), and thymol (4.5%), while nonacosane (42.5% and 27.2%), hexadecanoic acid (24.4% a . . .nd 20.0%), phytol (12.3%), and carvacrol (5.0%) were identified as the main components of D. oleoides subsp. oleoides obtained by hydrodistillation. Carvacrol (12.0%), thymol (7.7%), dihydroactinidiolide (7.2%), bicydosesquiphellandrene (5.5%), and (Z)-3-hexenal (4.1%) were the major components in D. pontica, while carvacrol (27.2% and 25.4%), nonacosane (24.6%), (Z)-3-hexenal (18.5% and 2.5%), decane (7.4%), hexahydrofarnesyl acetone (7.4% and 2.2%), hexanal (6.6% and 1.5%), heptacosane (6.1%), nonanal (5.6% and 1.9%), thymol (5.1% and 2.3%), and phytol (5.0%) were identified in the D. oleoides subsp. oleoides isolated by microdistillation, respectively. In addition, the volatile components were evaluated for their ability to scavenge 1,1-dipheny1-2-picrylhydrazyl (DPPH) radicals using a bioautographic thin layer chromatography (TLC) method, and the samples showed activity comparable with that of the tested standards, vitamins C and E Daha fazlası Daha az

Atay, I | Ilter, AZ | Bagatur, Y | Telci, D | Goren, AC | Kirmizibekmez, H | Yesilada, E

Conference Object | 2015 | PLANTA MEDICA81 ( 16 ) , pp.1512 - 1512

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Kirmizibekmez, H | Bardakci, H | Yesilada, E | Hohmann, J

Conference Object | 2011 | PLANTA MEDICA77 ( 12 ) , pp.1342 - 1343

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Aydemir, EA | Simsek, E | Imir, N | Gokturk, RS | Yesilada, E | Fiskin, K

Article | 2015 | PHARMACOGNOSY MAGAZINE11 ( 42 ) , pp.1342 - 1343

Background: Fabaceae family members are known to possess preventive and therapeutic potentials against various types of cancers. Objective: The aim of this study was to investigate the cytotoxic and apoptotic effects of hydroalcoholic extracts from the aerial parts and roots of an endemic Ebenus species; Ebenus boissieri Barbey in human lung cancer cell line. Materials and Methods: After treatment with hydroalcoholic extracts cytotoxic activities of both extracts were measured by 3-(4, 5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide assay, whereas caspase-3 activity, tumor necrosis factor-alpha (TNF-alpha) and interferon gam . . .ma (IFN-gamma) release were measured by enzyme linked immunosorbent assay. Results: According to in vitro assay results, the increase in all caspases activity suggested that extracts induce cells to undergo apoptosis. Especially, induction in caspase-3 activity was the most remarkable result of this study. Both aerial part and root extracts induced apoptosis by increasing caspase-3 activity, TNF-alpha and IFN-gamma release. When compared to their relative controls, the concentrations of both TNF-alpha and IFN-gamma in extract-treated groups were signi.cantly and dose dependently exalted. Conclusion: Taken together, our results indicate that the hydroalcoholic extracts of E. boissieri can be considered as a source of new anti-apoptotic and therefore anti-carcinogenic agent Daha fazlası Daha az

Ozer, G | Guzelmeric, E | Sezgin, G | Ozyurek, E | Arslan, A | Sezik, E | Yesilada, E

Article | 2018 | JOURNAL OF CHEMICAL METROLOGY12 ( 1 ) , pp.79 - 88

In this study, it was aimed to qualify the rhizome samples of butcher's broom (Ruscus aculeatus L.) gathered from various populations distributed naturally in The Marmara Region of Anatolia (Asian part of Turkey) based on the methods described in European Pharmacopoeia. For this purpose, rhizomes were sampled from 18 divers populations; namely, 6 from Istanbul, 4 from Bursa, 4 from Adapazari, 2 from Balikesir and 2 from Canakkale. For qualitative analysis HPTLC method was used in order identify ruscogenin and stigmasterol and these were determined in all samples. For quantitative analysis, concentrations of total ruscogenin (mixture . . . of neoruscogenin and ruscogenin) were analyzed by HPLC technique. According to The European Pharmacopoeia, Butcher's broom rhizomes should contain at least 1% ruscogenins and the rhizome samples obtained from Bahcekoy, Biga, Karacabey, Poyrazlar, Gonen and Kanlica were found consistent with this limit. For pilot field cultivation trial, rhizome sections were prepared from the selected rhizome samples by cutting carefully from their knuckles. The sections then were planted in the nursery under 3 different crown closures with three replications (one of which to serve control). However, it was observed that the survival ratio of seedlings, which was 1% in the first year in the nursery, declined even further in the 2nd and 3rd years, and finally the seedlings dried up, neither developed a root system nor formed a rhizome. Consequently, further cultivation studies should be carried out under suitable climatic conditions and soil compositions by directly planting the rhizomes Daha fazlası Daha az