Short-term oral magnesium supplementation suppresses bone turnover in postmenopausal osteoporotic women

Aydin, H. | Deyneli, O. | Yavuz, D. | Gözü, H. | Mutlu, N. | Kaygusuz, I. | Akalm, S.

Article | 2010 | Biological Trace Element Research133 ( 2 ) , pp.136 - 143

Magnesium has been shown to increase bone mineral density when used in the treatment of osteoporosis, yet its mechanism of action is obscure. In this study, the effects of daily oral magnesium supplementation on biochemical markers of bone turnover were investigated. Twenty postmenopausal women have been divided into two groups. Ten patients were given magnesium citrate (1,830 mg/day) orally for 30 days. Ten postmenopausal women of matching age, menopause duration, and BMI were recruited as the control group and followed without any medication. Fasting blood and first-void urine samples were collected on days 0, 1, 5, 10, 20, and 30 . . ., respectively. Total magnesium, calcium, phosphorus, iPTH and osteocalcin were determined in blood samples. Deoxypyridinoline levels adjusted for creatinine were measured in urine samples. Thirty consecutive days of oral magnesium supplementation caused significantly decrease in serum iPTH levels in the Mg-supplemented group ( Daha fazlası Daha az

Toxic metals in breast milk samples from Ankara, Turkey: Assessment of lead, cadmium, nickel, and arsenic levels

Gürbay, A. | Charehsaz, M. | Eken, A. | Sayal, A. | Girgin, G. | Yurdakök, M. | AydIn, A.

Article | 2012 | Biological Trace Element Research149 ( 1 ) , pp.117 - 122

Toxic metals are one of the significant groups of chemical contaminants that humans are exposed to by oral, inhalation, and dermal routes. Exposure to these chemicals begins with intrauterine life and continues during lactation period at the first years of life. Breastfeeding has a much more special place than other nutrition options for infants. However, when possibility of contaminant transfer by breast milk is considered, its safety and quality is essential. Regarding infant and mother health and limited number of information on this field in Turkey, measuring contamination levels in breast milk is important. Therefore, in the pr . . .esent study, lead (Pb), cadmium (Cd), nickel (Ni), and arsenic (As) levels were measured by atomic absorption spectrometry in 64 breast milk samples obtained from mothers from Ankara, Turkey. Pb and Ni levels in breast milk samples were found to be 391.45 ± 269.01 µg/l and 43.94 ± 33.82 µg/l (mean ± SD), respectively. Cd was found only in one of 64 samples, and the level was 4.62 µg/l. As level was below the limit of quantification (LOQ, 7.6 µg/l) in all samples. These findings will accurately direct strategies and solutions of protection against contaminants in order to reduce their levels in biological fluids. © 2012 Springer Science+Business Media, LLC Daha fazlası Daha az

Effects of Intermittent and Continuous Magnetic Fields on Trace Element Levels in Guinea Pigs

Erdem, O. | Akay, C. | Cevher, S.C. | Canseven, A.G. | Aydın, A. | Seyhan, N.

Article | 2018 | Biological Trace Element Research181 ( 2 ) , pp.265 - 271

Electromagnetic fields (EMFs) can affect living cells due to biochemical changes, followed by changes in levels of trace elements in serum and different organs. This study focuses on the effect of whole body exposure to EMF, presented everywhere in our environment, and on the levels of trace elements in serum, femur, brain, kidney, and liver tissues. The analyses performed on 29 guinea pigs were divided into five groups. Guinea pigs were exposed to a magnetic field of 50 Hz of 1.5 mT. Groups A and B were exposed to the magnetic field for a period of 4 h/day continuously (4 h/day) for 4 and 7 days, respectively. Groups C and D were e . . .xposed to the magnetic field for a period of 4 h/day intermittently for 4 and 7 days, respectively. Group E animals were enrolled as control. Copper (Cu), zinc (Zn), calcium (Ca), and magnesium (Mg) levels were determined by atomic absorption spectroscopy in serum, femur, brain, kidney, and liver tissues in all guinea pigs. When compared to the control groups, the changes in the levels of Cu in serum samples, femur, and kidney tissues of the treated groups were statistically significant. The same was also true for the levels of Mg in the brain, kidney, and lung tissues. Our results suggest that in vivo continuous and intermittent exposure to EMF may cause disturbances in homeostasis of bioelements. These effects could be important risk factors for toxic effects of EMF, especially in relation to deterioration of bioelements. © 2017, Springer Science+Business Media New York Daha fazlası Daha az

A pilot study on neopterin levels and tryptophan degradation in zinc-exposed galvanization workers

Sarac, E.S. | Girgin, G. | Palabiyik, S.S. | Charehsaz, M. | Aydin, A. | Sahin, G. | Baydar, T.

Article | 2013 | Biological Trace Element Research151 ( 3 ) , pp.330 - 334

Hot-dip galvanization is a zinc-coating process to protect the metal items from corrosion. Zinc oxide nanoaerosol fume rising from hot metal bath surface in nano dimensions contains the greatest risk for workers in galvanization process. In the present study, it was evaluated whether inhalation of zinc causes any alteration in cellular immunity and tryptophan degradation by measuring neopterin, tryptophan, kynurenine, and zinc levels in 63 male galvanization workers and 23 male office personnel as controls. Serum and urinary zinc levels were found as 14.90 ± 0.90 and 102 ± 4.7 µg/dL in workers while 12.87 ± 1.45 and 75 ± 4.2 µg/dL i . . .n controls, respectively (both, p< 0.05). Similarly, the mean urinary neopterin levels and serum neopterin and kynurenine levels were found to be statistically higher in galvanization workers than the controls (all, p< 0.05). Significant correlations were found between urinary neopterin levels and kynurenine to tryptophan ratio or serum zinc levels. The results indicated cellular immune activation by occupational zinc exposure. It was estimated that neopterin, in parallel with kynurenine pathway, could reflect occupational exposure to zinc nanoaerosols and might be useful in early diagnosis of immune alterations due to nano-scale exposures. © Springer Science+Business Media New York 2012 Daha fazlası Daha az

Decreased serum selenium levels are correlated with diminished coronary flow reserve among hemodialysis patients

Atakan, A. | Macunluoglu, B. | Kaya, Y. | Ari, E. | Demir, H. | Asicioglu, E. | Kaspar, C.

Article | 2013 | Biological Trace Element Research155 ( 3 ) , pp.333 - 338

Cardiovascular diseases are the main reason of high mortality among hemodialysis patients. Decreased serum selenium levels may have a role in accelerated atherosclerosis in this patient group. The hypothesis of this study was to show a correlation between decreased serum selenium levels and coronary flow reserve as an indicator of endothelial dysfunction and atherosclerosis in HD patients. Seventy-one chronic hemodialysis patients and age 65 and sex-matched healthy controls were included in the study. Plasma selenium levels were measured by spectrophotometry, and coronary flow reserve was assessed by transthoracic Doppler echocardio . . .graphy. Serum selenium levels (34.16 ± 6.15 ng/ml vs. 52.4 ± 5.51 ng/ml, P < 0.001) and coronary flow reserve values (1.73 ± 0.11 vs. 2.32 ± 0.28, P < 0.001) were significantly lower in hemodialysis patients compared with controls, respectively. There was a significant positive correlation between coronary flow reserve and serum levels of selenium (r = 0.676, P < 0.001). A linear regression analysis showed that serum levels of selenium were independently and positively correlated with coronary flow reserve (regression coefficient = 0.650, P < 0.05). This study was the first to show a positive and independent correlation between decreased selenium levels and diminished coronary flow reserve as an indicator of endothelial dysfunction and atherosclerosis in hemodialysis patients. Our data suggest that decreased serum selenium levels may facilitate the development of endothelial dysfunction and disruption of coronary flow reserve which occur before the development of overt atherosclerosis. © 2013 Springer Science+Business Media New York Daha fazlası Daha az

Boron Content of Some Foods Consumed in Istanbul, Turkey

Kuru, R. | Yilmaz, S. | Tasli, P.N. | Yarat, A. | Şahin, Fikrettin

Article | 2019 | Biological Trace Element Research187 ( 1 ) , pp.333 - 338

The boron content was determined in 42 different foods consumed in Istanbul, Turkey. Eleven species of fruit, ten species of vegetable, eight species of food of animal origin, four species of grain, two species of nuts, two species of legume, and five other kinds of foods were included to this study. They were analyzed by two methods: Inductively coupled plasma mass spectrometry (ICP-MS) technique and carminic acid assay, and the results of two methods were also compared. Boron concentration in foods ranged between 0.06–37.2 mg/kg. Nuts had the highest boron content while foods of animal origin had the lowest. A strong correlation w . . .as found between the results of the carminic acid assay and the ICP-MS technique (p = 0.0001, Pearson correlation coefficient: r = 0.956). Bland Altman analysis also supported this correlation. ICP-MS is one of the most common, reliable, and powerful method for boron determination. The results of our study show that spectrophotometric carminic acid assay can provide similar results to ICP-MS, and the boron content in food materials can be also determined by spectrophotometric method. © 2018, Springer Science+Business Media, LLC, part of Springer Nature Daha fazlası Daha az

Erratum to: A pilot study on neopterin levels and tryptophan degradation in zinc-exposed galvanization workers (Biological Trace Element Research (2013) 151, (330-334) DOI: 10.1007/s12011-012-9569-4)

Sarac, E.S. | Girgin, G. | Palabiyik, S.S. | Charehsaz, M. | Aydin, A. | Sahin, G. | Baydar, T.

Erratum | 2014 | Biological Trace Element Research158 ( 1 ) , pp.333 - 338

[No abstract available]

The effects of zinc treatment on the blood-brain barrier permeability and brain element levels during convulsions

Yorulmaz, H. | Şeker, F.B. | Demir, G. | Yalçin, I.E. | Öztaş, B.

Article | 2013 | Biological Trace Element Research151 ( 2 ) , pp.256 - 262

We evaluated the effect of zinc treatment on the blood-brain barrier (BBB) permeability and the levels of zinc (Zn), natrium (Na), magnesium (Mg), and copper (Cu) in the brain tissue during epileptic seizures. The Wistar albino rats were divided into four groups, each as follows: (1) control group, (2) pentylenetetrazole (PTZ) group: rats treated with PTZ to induce seizures, (3) Zn group: rats treated with ZnCl2 added to drinking water for 2 months, and (4) Zn + PTZ group. The brains were divided into left, right hemispheres, and cerebellum + brain stem regions. Evans blue was used as BBB tracer. Element concentrations were analyzed . . . by inductively coupled plasma optical emission spectroscopy. The BBB permeability has been found to be increased in all experimental groups (p < 0.05). Zn concentrations in all brain regions in Zn-supplemented groups (p < 0.05) showed an increase. BBB permeability and Zn level in cerebellum + brain stem region were significantly high compared to cerebral hemispheres (p < 0.05). In all experimental groups, Cu concentration decreased, whereas Na concentrations showed an increase (p < 0.05). Mg content in all the brain regions decreased in the Zn group and Zn + PTZ groups compared to other groups (p < 0.001). We also found that all elements' levels showed hemispheric differences in all groups. During convulsions, Zn treatment did not show any protective effect on BBB permeability. Chronic Zn treatment decreased Mg and Cu concentration and increased Na levels in the brain tissue. Our results indicated that Zn treatment showed proconvulsant activity and increased BBB permeability, possibly changing prooxidant/antioxidant balance and neuronal excitability during seizures. © 2012 The Author(s) Daha fazlası Daha az

Boron enhances odontogenic and osteogenic differentiation of human tooth germ stem cells (hTGSCs) in vitro

Taşlı, P.N. | Doğan, A. | Demirci, S. | Şahin, Fikrettin

Article | 2013 | Biological Trace Element Research153 ( 01.03.2020 ) , pp.419 - 427

Stem cell technology has been a great hope for the treatment of many common problems such as Parkinson's disease, Alzheimer's disease, diabetes, cancer, and tissue regeneration. Therefore, the main challenge in hard tissue engineering is to make a successful combination of stem cells and efficient inductors in the concept of stem cell differentiation into odontogenic and osteogenic cell types. Although some boron derivatives have been reported to promote bone and teeth growth in vivo, the molecular mechanism of bone formation has not been elucidated yet. Different concentrations of sodium pentaborate pentahydrate (NaB) were prepared . . . for the analysis of cell toxicity and differentiation evaluations. The odontogenic, osteogenic differentiation and biomineralization of human tooth germ stem cells (hTGSCs) were evaluated by analyzing the mRNA expression levels, odontogenic and osteogenic protein expressions, alkaline phosphatase (ALP) activity, mineralization, and calcium deposits. The NaB-treated group displayed the highest ALP activity and expression of osteo- and odontogenic-related genes and proteins compared to the other groups and baseline. In the current study, increased in vitro odontogenic and osteogenic differentiation capacity of hTGSCs by NaB application has been shown for the first time. The study offers considerable promise for the development of new scaffold systems combined with NaB in both functional bone and tooth tissue engineering. © 2013 Springer Science+Business Media New York Daha fazlası Daha az

Evaluation the Weekly Intake of Some Wild Edible Indigenous Mushrooms Collected in Different Regions in Tunceli, Turkey

Alp, H. | Ince, M. | Ince, O.K. | Onal, A.

Article | 2019 | Biological Trace Element Research , pp.419 - 427

The quantity of some essential and non-essential elements of wild edible mushroom samples collected from Tunceli Province of Turkey was determined by using flame and electrothermal atomic absorption spectrometer after microwave digestion. The method accuracy was corrected using standard reference material (NIST SRM 1547-Peach Leaves). The essential element concentrations of analyzed mushroom samples were determined in the range of 0.036–0.563 mg kg-1 for calcium, 1.28–2.55 mg kg-1 for magnesium, 0.054–0.188 mg kg-1 for sodium, 1.00–4.57 mg kg-1 for copper, 212–480 mg kg-1 for iron, and 75–151 mg kg-1 for zinc but cobalt and chromium . . . were not detected. Based on results, there were statistically significant differences between the element contents of analyzed mushroom species. Consequently, according to this study results, the weekly intake and target hazard quotient values of the elements show that the consumption of these mushrooms does not threaten human health. [Figure not available: see fulltext.]. © 2019, Springer Science+Business Media, LLC, part of Springer Nature Daha fazlası Daha az

Dose-dependent Effect of Boric Acid on Myogenic Differentiation of Human Adipose-derived Stem Cells (hADSCs)

Apdik, H. | Doğan, A. | Demirci, S. | Aydın, S. | Şahin, Fikrettin

Article | 2015 | Biological Trace Element Research165 ( 2 ) , pp.123 - 130

Boron, a vital micronutrient for plant metabolism, is not fully elucidated for embryonic and adult body development, and tissue regeneration. Although optimized amount of boron supplement has been shown to be essential for normal gestational development in zebrafish and frog and beneficial for bone regeneration in higher animals, effects of boron on myogenesis and myo-regeneration remains to be solved. In the current study, we investigated dose-dependent activity of boric acid on myogenic differentiation of human adipose-derived stem cells (hADSCs) using immunocytochemical, gene, and protein expression analysis. The results revealed . . . that while low- (81.9 µM) and high-dose (819.6 µM) boron treatment increased myogenic gene expression levels such as myosin heavy chain (MYH), MyoD, myogenin, and desmin at day 4 of differentiation, high-dose treatment decreased myogenic-related gene and protein levels at day 21 of differentiation, confirmed by immunocytochemical analysis. The findings of the study present not only an understanding of boron’s effect on myogenic differentiation but also an opportunity for the development of scaffolds to be used in skeletal tissue engineering and supplements for embryonic muscle growth. However, fine dose tuning and treatment period arranging are highly warranted as boron treatment over required concentrations and time might result in detrimental outcomes to myogenesis and myo-regeneration. © 2015, Springer Science+Business Media New York Daha fazlası Daha az

Suppressor Effects of Sodium Pentaborate Pentahydrate and Pluronic F68 on Adipogenic Differentiation and Fat Accumulation

Yilmaz, A.B. | Tapsin, S. | Elbasan, E.B. | Kayhan, H.D. | Şahin, Fikrettin | Turkel, N.

Article | 2020 | Biological Trace Element Research193 ( 2 ) , pp.390 - 399

Obesity is a major public health problem worldwide and a risk factor for certain diseases, including cardiovascular disease, diabetes, cancer, and depression. Unfortunately, currently available anti-obesity drugs have failed in the long-term maintenance of weight control. It has been a challenge to design novel drugs that could potentially treat obesity or prevent uncontrolled weight-gain which lies underneath the pathology of obesity. Since obesity in a way is a consequence of the accumulating new mature adipocytes from undifferentiated precursors which is a process also termed as adipogenesis, drugs that might control adipogenesis . . . could be beneficial for the treatment of obesity. In the current study, combined effect of sodium pentaborate pentahydrate (NaB) and pluronic F68 on adipogenic differentiation was examined by administering various combinations of the two agents to human adipose-derived stem cells (hADSCs) in in vitro. Immunocytochemistry and quantitative RT-PCR were performed to evaluate the levels of adipogenesis-promoting genes such as peroxisome proliferator-activated receptor-? (PPAR?), fatty acid binding protein (FABP4), and adiponectin. Results indicated that expressions of all these three genes were restrained. Furthermore, Oil Red O staining revealed that lipid vesicle formation was reduced in hADSCs treated with differentiation medium containing NaB/F68 combination. Finally, expression levels of Hippo pathway kinases Lats2, MST1, and scaffold protein Sav1 were reduced in these cells, suggesting a possible link between Hippo pathway-dependent downregulation of PPAR? and the NaB/F68 treatment. Herein, we showed that combination of NaB and F68 curtails adipocyte differentiation by inhibiting the adipogenic transcriptional program leading to a decrease in lipid accumulation in adipocytes even at very low doses, thereby uncovered a striking opportunity to use this combination in obesity treatment. © 2019, Springer Science+Business Media, LLC, part of Springer Nature Daha fazlası Daha az

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