Margunato, S | Tasli, PN | Aydin, S | Kazandag, MK | Şahin, Fikrettin

Article | 2015 | JOURNAL OF ENDODONTICS41 ( 10 ) , pp.1646 - 1652

Introduction: Stem cell technology has been a great hope for the regeneration of cells of pulp-dentin complex and dental structures together with surrounding bone and periodontium. The main challenge in the regeneration process is a successful combination of stem cells and efficient inductors such as inductive bio-materials. In this regard, today, manufacturers propose novel tooth filling materials. The current study was aimed to compare the effect of Pro Root MTA (Dentsply Tulsa Dental, Tulsa, OK), Biodentine (Septodont, Saint Maur des Fosses, France), and MM-MTA (Micro-Mega, Besancon Cedex, France) on the cell viability, hard tiss . . .ue deposition capacity, and osteogenic differentiation of human bone marrow stem cells (hBMSCs) derived from mandibular bone. Methods: Dental materials were packed into Teflon rings (Grover Corp, Milwaukee, WI) and placed on Transwell inserts (Corning, Corning, NY) to determine the toxicity of tooth filling materials by the 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium assay on days 1, 3, 7, and 14; 20% dimethyl sulfoxide (DMSO) was used as a positive control for the toxicity assay. hBMSCs were characterized by their surface markers with mesenchymal stem cell antibodies. Teflon rings were cocultured with hBMSCs followed by the induction of osteogenic differentiation. The osteogenic differentiation of hBMSCs and hard tissue formation of the materials were evaluated by analyzing the messenger RNA expression levels of osteonectin, Runt-related transcription factor 2, and collagen type, 1A by real-time polymerase chain reaction expression analysis, measurement of alkaline phosphatase activity, and visualization of calcium deposits by alizarin red staining. Results: MTA, Biodentine, and MM-MTA did not exhibit a cytotoxic effect on hBMSCs after 14 days in culture. Even though all the materials significantly stimulate (P < .05) osteogenic differentiation of hBMSCs compared with the negative control, ProRoot MTA showed greater osteoinductivity than Biodentine or MM-MTA according to the messenger RNA expression, alkaline phosphatase, immunocytochemistry, and alizarin red staining data. Conclusions: All of the dental materials used in this study show the osteogenic differentiation potential of hBMSCs. Therefore, newly introduced MM-MTA can also be used as a material of choice in routine dental treatment Daha fazlası Daha az

Kulan, P | Karabiyik, O | Kose, GT | Kargul, B

Article | 2016 | JOURNAL OF ENDODONTICS42 ( 2 ) , pp.276 - 279

The aim of this study was to evaluate the effects of several additives on the setting time and cytotoxicity of accelerated-set mineral trioxide aggregate (MTA) on stem cells of human dental pulp. ProRoot white MTA (WMTA) (Dentsply Tulsa Dental, Johnson City, TN) was mixed with various additives including distilled water, 2.5% disodium hydrogen phosphate (Na2HPO4) (Merck, Darmstadt, Germany), K-Y Jelly (Johnson & Johnson, Markham, ON, Canada), and 5% and 10% calcium chloride (CaCl2) (Merck). The setting times were evaluated using a Vicat apparatus (Alsa Lab, Istanbul, Turkey). Human dental pulp stem cells were isolated and seeded int . . .o 48-well plates at 2 x 10(3) cells per well and incubated with MTA samples for 24 hours, 3 days, and 7 days. Cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. MTA mixed with 10% CaCl2 showed the lowest setting time (P < .05). According to the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sullophenyl)-2H-tetrazolium results on the 1st, 3rd, and 7th days, a statistically significant difference was found (P < .05) between MTA groups and the control group. MTA mixed with K-Y Jelly in all groups showed the lowest cell viability at all time points (P < .05). The cell viability of MTA mixed with distilled water, 5% CaCl2, 10% CaCl2, and Na2HPO4 increased significantly through time (P < .05). This in vitro study found MTA mixed with 5% and 10% CaCl2 and Na2HPO4 is biocompatible with dental pulp stem cells in terms of cell viability. Further in vitro and in vivo investigations are required to prove the clinical applications of MTA mixed with various additives Daha fazlası Daha az

Kocaelli, HA | Kaptan, RF | Kayahan, B | Haznedaroglu, F

Correction | 2007 | JOURNAL OF ENDODONTICS33 ( 1 ) , pp.68 - 68

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