Effect of vascular endothelial growth factor on sperm motility and survival
Vascular endothelial growth factor (VEGF) and its receptors are present in both male and female reproductive systems. In this experimental study, the effect of different concentrations of VEGF on sperm motility and survival in vitro was investigated. Human spermatozoa, collected from voluntary, proven fertile donors, were incubated in sperm washing medium containing different concentrations of VEGF (5, 10, 15, 20 ng/ml) for 24 h in a university reproductive endocrinology laboratory setting. Assessment of VEGF action on sperm motion characteristics was evaluated using a computer-assisted semen analyser. Sperm survival was determined by hypo-osmotic swelling and eosin-Y dye tests. VEGF had a positive effect on some parameters of sperm motility in a concentration-dependent manner. Maximal effect was observed at a concentration of 15 ng/ml; motility, progression, straight-line velocity and curvilinear velocity of VEGF-exposed spermatozoa were significantly increased (P < 0.05) at this concentration. However, sperm viability was not prolonged at any concentration of VEGF as shown by hypo-osmotic swelling and eosin-Y dye tests. VEGF may increase some sperm motility parameters, but not survival, in a concentration-dependent manner in vitro. © 2009 Published by Reproductive Healthcare Ltd.
| Yazar |
Iyibozkurt, A.C. Balcik, P. Bulgurcuoglu, S. Arslan, B.K. Attar, Rukset Attar, E. |
| Yayın Türü | Article |
| Tek Biçim Adres | https://hdl.handle.net/20.500.11831/3579 |
| Konu Başlıkları |
CASA
Fallopian tube Infertility Male factor Spermatozoa VEGF |
| Koleksiyonlar |
Araştırma Çıktıları | Ön Baskı | WoS | Scopus | TR-Dizin | PubMed 02- WoS İndeksli Yayınlar Koleksiyonu 03- Scopus İndeksli Yayınlar Koleksiyonu 05- PubMed İndeksli Yayınlar Koleksiyonu |
| Dergi Adı | Reproductive BioMedicine Online |
| Cild | 19 |
| Dergi Sayısı | 6 |
| Sayfalar | 784 - 788 |
| Yayın Tarihi | 2009 |
| Eser Adı [dc.title] | Effect of vascular endothelial growth factor on sperm motility and survival |
| Yazar [dc.contributor.author] | Iyibozkurt, A.C. |
| Yazar [dc.contributor.author] | Balcik, P. |
| Yazar [dc.contributor.author] | Bulgurcuoglu, S. |
| Yazar [dc.contributor.author] | Arslan, B.K. |
| Yazar [dc.contributor.author] | Attar, Rukset |
| Yazar [dc.contributor.author] | Attar, E. |
| Yayıncı [dc.publisher] | Elsevier Ltd |
| Yayın Türü [dc.type] | article |
| Özet [dc.description.abstract] | Vascular endothelial growth factor (VEGF) and its receptors are present in both male and female reproductive systems. In this experimental study, the effect of different concentrations of VEGF on sperm motility and survival in vitro was investigated. Human spermatozoa, collected from voluntary, proven fertile donors, were incubated in sperm washing medium containing different concentrations of VEGF (5, 10, 15, 20 ng/ml) for 24 h in a university reproductive endocrinology laboratory setting. Assessment of VEGF action on sperm motion characteristics was evaluated using a computer-assisted semen analyser. Sperm survival was determined by hypo-osmotic swelling and eosin-Y dye tests. VEGF had a positive effect on some parameters of sperm motility in a concentration-dependent manner. Maximal effect was observed at a concentration of 15 ng/ml; motility, progression, straight-line velocity and curvilinear velocity of VEGF-exposed spermatozoa were significantly increased (P < 0.05) at this concentration. However, sperm viability was not prolonged at any concentration of VEGF as shown by hypo-osmotic swelling and eosin-Y dye tests. VEGF may increase some sperm motility parameters, but not survival, in a concentration-dependent manner in vitro. © 2009 Published by Reproductive Healthcare Ltd. |
| Kayıt Giriş Tarihi [dc.date.accessioned] | 2020-03-18 |
| Yayın Tarihi [dc.date.issued] | 2009 |
| Açık Erişim Tarihi [dc.date.available] | 2020-03-18 |
| Dil [dc.language.iso] | eng |
| Konu Başlıkları [dc.subject] | CASA |
| Konu Başlıkları [dc.subject] | Fallopian tube |
| Konu Başlıkları [dc.subject] | Infertility |
| Konu Başlıkları [dc.subject] | Male factor |
| Konu Başlıkları [dc.subject] | Spermatozoa |
| Konu Başlıkları [dc.subject] | VEGF |
| Haklar [dc.rights] | info:eu-repo/semantics/openAccess |
| ISSN [dc.identifier.issn] | 14726483 |
| Yayının ilk sayfa sayısı [dc.identifier.startpage] | 784 |
| Yayının son sayfa sayısı [dc.identifier.endpage] | 788 |
| Dergi Adı [dc.relation.journal] | Reproductive BioMedicine Online |
| Dergi Sayısı [dc.identifier.issue] | 6 |
| Cild [dc.identifier.volume] | 19 |
| Tek Biçim Adres [dc.identifier.uri] | https://hdl.handle.net/20.500.11831/3579 |
| Pubmed Id [dc.identifier.pubmed] | PubMed ID: 20031017 |
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